Zinc finger of Arabidopsis thaliana 6 is involved in melatonin-mediated auxin signaling through interacting INDETERMINATE DOMAIN15 and INDOLE-3-ACETIC ACID 17
Zinc finger of Arabidopsis thaliana 6 is involved in melatonin-mediated auxin signaling through interacting INDETERMINATE DOMAIN15 and INDOLE-3-ACETIC ACID 17
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拟南芥 6 的锌指通过相互作用的不确定结构域 15 和 INDOLE-3-ACETIC ACID 17 参与褪黑素介导的生长素信号传导
DOI：
10.1111/jpi.12494
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发表时间：
2018
影响因子：
10.3
通讯作者：
Chan Zhulong
中科院分区：
文献类型：
--
作者：
Shi Haitao;Zhang Shengmin;Lin Daozhe;Wei Yunxie;Yan Yu;Liu Guoyin;Reiter Russel J;Chan Zhulong
虽然越来越多的证据表明褪黑激素和生长素作为色氨酸的衍生物之间的串扰，潜在的信号事件仍然不清楚。本研究发现，褪黑素和生长素以相互拮抗的方式介导拟南芥锌指蛋白（ZAT 6）的转录水平，ZAT 6负调控内源生长素水平，ZAT 6基因敲除的植物对褪黑素调控的生长素合成不敏感，表明其参与褪黑素介导的生长素积累。此外，在体内与ZAT 6相互作用的吲哚乙酸结构域15（IDD 15）和吲哚-3-乙酸17（IAA 17）的鉴定为ZAT 6介导的生长素信号转导提供了新的见解。进一步的研究表明，ZAT 6抑制IDD 15在YUC 2启动子上的转录激活，而ZAT 6通过竞争性结合IAA 17，抑制运输抑制剂反应1（TIR 1）与IAA 17的相互作用。因此，生长素的合成和生长素的反应都受到ZAT 6的负调控。综上所述，ZAT 6在拟南芥中通过形成生长素信号通路的相互作用复合物参与褪黑激素介导的生长素信号通路。
Although accumulating evidence demonstrates the cross talk between melatonin and auxin as derivatives of tryptophan, the underlying signaling events remain unclear. In this study, we found that melatonin and auxin mediated the transcriptional levels ofzinc finger of Arabidopsis thaliana(ZAT6) in a mutually antagonistic manner.ZAT6negatively modulated the endogenous auxin level, andZAT6knockdown plants were less sensitive to melatonin‐regulated auxin biosynthesis, indicating its involvement in melatonin‐mediated auxin accumulation. Additionally, the identification of INDETERMINATE DOMAIN15 (IDD15) and INDOLE‐3‐ACETIC ACID 17 (IAA17) inArabidopsisthat interacted with ZAT6 in vivo provided new insight ofZAT6‐mediated auxin signaling. Further investigation showed that ZAT6 repressed the transcription activation of IDD15 on theYUC2promoter, while ZAT6 inhibited the interaction of TRANSPORT INHIBITOR RESPONSE 1 (TIR1) and IAA17 through competitively binding to IAA17. Thus, both auxin synthesis and the auxin response were negatively modulated byZAT6. Taken together,ZAT6is involved in melatonin‐mediated auxin signaling through forming an interacting complex of auxin signaling pathway inArabidopsis.