Primary structure, developmentally regulated expression and potential duplication of the zebrafish homeobox gene ZF-21.
Primary structure, developmentally regulated expression and potential duplication of the zebrafish homeobox gene ZF-21.
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斑马鱼同源盒基因 ZF-21 的一级结构、发育调控表达和潜在重复。
DOI：
10.1093/nar/16.19.9097
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发表时间：
1988
影响因子：
14.9
通讯作者：
A. Fjose
中科院分区：
文献类型：
--
作者：
Pål R. Njølstad;A. Molven;Ivar Hordvik;Jaran Apold;A. Fjose
我们报告了源自斑马鱼基因 (ZF-21) 的 cDNA 的分子克隆和表征，该基因与含有基因 Hox2.1 的小鼠同源盒相关。通过比较 ZF-21（275 个氨基酸）和 Hox2.1（279 个氨基酸）的推定蛋白质序列，获得了有关各个结构域差异保守性的有趣信息。包含 ZF-21 同源域和 36 个额外侧翼残基的独立 DNA 结合域与 Hox2.1 的 C 端部分完全相同。因此，这两种小鼠和斑马鱼蛋白必须具有相同的 DNA 结合特性。 ZF-21 和 Hox2.1 N 端编码区之间较低水平的序列同一性将总蛋白同源性降低至 81%。然而，这些 N 末端的短距离完美同源性表明基本的生化功能是相同的。正如对真正的同源物所预期的那样，ZF-21 和 Hox2.1 基因在胚胎发生过程中的非编码序列和时间表达方面也具有广泛的相似性。潜在 ZF-21 重复的发现与脊椎动物同源框基因的功能和进化方面进行了讨论。
We report the molecular cloning and characterization of a cDNA derived from a zebrafish gene (ZF-21) related to the mouse homeobox containing gene Hox2.1. Interesting information about the differential conservation of various domains was gained from comparisons between the putative protein sequences from ZF-21 (275 amino acids) and Hox2.1 (279 aa). A separate DNA binding domain including the ZF-21 homeodomain and 36 additional flanking residues is completely identical to the C-terminal part of Hox2.1. As a consequence, these two mouse and zebrafish proteins must have identical DNA binding properties. A lower level of sequence identity between the N-terminal coding regions of ZF-21 and Hox2.1 reduces the total protein homology to 81%. However, short stretches of perfect homology in these N-terminals suggests that the essential biochemical functions are the same. As expected for true homologues, the ZF-21 and Hox2.1 genes also share extensive similarities with respect to non-coding sequences and temporal expression during embryogenesis. The finding of a potential ZF-21 duplication is discussed in relation to functional and evolutionary aspects of vertebrate homeobox genes.