Anatomy of plasmid DNAs with anti-silencing elements
Anatomy of plasmid DNAs with anti-silencing elements
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具有抗沉默元件的质粒 DNA 的解剖
DOI：
10.1016/j.ijpharm.2014.01.025
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
H. Harashima and H. Kamiya
中科院分区：
文献类型：
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作者：
G. N. Kanda;S. Miyamoto;M. Kobayashi;I. Matsuoka;H. Harashima and H. Kamiya
转基因表达的瞬时性是发展非病毒载体的主要障碍。据报道，无CpG和pLIVE质粒在小鼠肝脏中实现了长期转基因表达。在这项工作中，这些质粒内的抗沉默元件进行了研究。还检查了被沉默的质粒对来自无CpG质粒的转基因表达的影响以及在早期时间点转基因表达对沉默的影响。结果表明，pLIVE质粒中白蛋白基因的3′端非翻译区和无CpG基序质粒的骨架序列有助于其持久表达。此外，未检测到另一质粒的沉默对无CpG质粒表达或转基因表达在沉默的早期时间点的持续时间的影响。
The transience of transgene expression is a major obstacle in the development of nonviral vectors. The CpG-free and pLIVE plasmids reportedly achieve long-term transgene expression in mouse liver. In this work, the anti-silencing elements within these plasmids were studied. The effects of plasmid that was being silenced on transgene expression from the CpG-free plasmid and those of transgene expression at early time points on silencing were also examined. The results suggested that the backbone sequence of the CpG-free plasmid and the 3′ untranslated region of the albumin gene of the pLIVE plasmid contribute to durable expression. In addition, no influence of the silencing of another plasmid on the duration of CpG-free plasmid expression or of transgene expression at early time points on silencing was detected.