Distribution of tccP in clinical enterohemorrhagic and enteropathogenic Escherichia coli isolates
Distribution of tccP in clinical enterohemorrhagic and enteropathogenic Escherichia coli isolates
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DOI：
10.1128/jcm.43.11.5715-5720.2005
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发表时间：
2005-11-01
影响因子：
9.4
通讯作者：
Frankel, G
中科院分区：
文献类型：
--
作者：
Garmendia, J;Ren, ZH;Frankel, G
肠出血性大肠杆菌（EHEC）和肠致病性大肠杆菌。大肠杆菌（EPEC）是通过形成附着和消退病变而定殖于肠道的病原体，其依赖于效应蛋白经由肠上皮细胞消退编码的III型分泌系统的位点的移位。最近，EHEC O 157：H7原噬菌体CP-933 U上相邻基因编码的两个效应蛋白EspJ和TccP已被鉴定。TccP由一个独特的N-末端区域和几个富含脯氨酸的结构域组成。在本项目中，我们确定了O 157：H7、非O 157 EHEC以及典型和非典型EPEC分离株中tccP的分布。所有检测的EHEC O 157：H7菌株均为tccP（+）。出乎意料的是，在非O 157 EHEC、典型和非典型EPEC分离株中也发现了tccP，特别是属于血清群O26（EHEC）、O 119（典型EPEC）和O 55（非典型EPEC）的菌株。我们记录了tccP长度的一些变化，这反映了富含脯氨酸的重复序列数量的多样性。这些结果表明存在一类“附着和消失”的病原体，它们表达EPEC和EHEC毒力决定因子的组合。
Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) are diarrheagenic pathogens that colonize the gut through the formation of attaching and effacing lesions, which depend on the translocation of effector proteins via a locus of enterocyte effacement-encoded type III secretion system. Recently, two effector proteins, EspJ and TccP, which are encoded by adjacent genes on prophage CP-933U in EHEC O157:H7, have been identified. TccP consists of a unique N-terminus region and several proline-rich domains. In this project we determined the distribution of tccP in O157:H7, in non-O157 EHEC, and in typical and atypical EPEC isolates. All the EHEC O157:H7 strains tested were tccP(+). Unexpectedly, tccP was also found in non-O157 EHEC, and in typical and atypical EPEC isolates, particularly in strains belonging to serogroups O26 (EHEC), O119 (typical EPEC), and O55 (atypical EPEC). We recorded some variation in the length of tccP, which reflects diversity in the number of the proline-rich repeats. These results show the existence of a class of "attaching and effacing" pathogens which express a combination of EPEC and EHEC virulence determinants.