Positron emission tomography combined with computed tomography finding: low glucose metabolism in hepatocellular carcinoma with GPC3 expression
Positron emission tomography combined with computed tomography finding: low glucose metabolism in hepatocellular carcinoma with GPC3 expression
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正电子发射断层扫描联合计算机断层扫描发现：肝细胞癌低糖代谢及GPC3表达
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影响因子：
4.3
通讯作者：
Hu-Bing Wu
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作者：
You-Cai Li;Chuan-Sheng Yang;Wen-Lan Zhou;Hong-Sheng Li;Yan-Jiang Han;Quan-Shi Wang;Hu-Bing Wu
IM：研究表达（磷脂酰肌醇蛋白聚糖-3）GPC 3的肝细胞癌中的葡萄糖代谢。方法：对55例新诊断的HCC患者进行了GPC 3和葡萄糖转运蛋白1（GLUT1）表达的病理样本的免疫组织化学染色，以及用于测量肿瘤葡萄糖摄取的全身18F-FDG PET/CT。采用最大标准摄取值（SUVmax）和肿瘤与非肿瘤肝摄取（T/NT）比值定量18F-FDG摄取。体外18F-FDG摄取实验检测GPC3对HepG2和非GPC3表达的RH7777细胞葡萄糖代谢的影响。采用斯皮尔曼等级相关、单因素和多因素Logistic回归分析GPC3表达与18 F-FDG摄取、GLUT1表达、肿瘤分化程度等临床指标的关系。结果如下：GPC3在67.3%的HCC患者中呈阳性表达，其中高分化或中分化HCC患者为75.0%，低分化HCC患者为36.4%。GPC 3表达与SUVmax呈负相关（斯皮尔曼相关系数=-0.281，P = 0.038），GLUT 1表达与SUVmax呈正相关（斯皮尔曼相关系数= 0.681，P 0.05）。进一步多因素分析显示，只有T/N比值与HCC患者GPC3表达显著相关（P <0. 05）。体外实验显示，表达GPC 3的HepG2细胞摄取18F-FDG的能力明显低于不表达GPC 3的RH7777细胞（t =-20.352，P <0.001）。结论：目前的研究表明，GPC3的表达与糖代谢呈负相关，提示GPC3可能在HCC中起调节糖代谢的作用。
IM: To investigate the glucose metabolism in hepatocellular carcinomas expressing (glypican-3) GPC3. .METHODS: Immunohistochemical staining of pathological samples for GPC3 and glucose transporter 1 (GLUT1) expression, and whole-body 18F-FDG PET/CT for measuring tumour glucose uptake were performed in 55 newly diagnosed HCC patients. The maximum standard uptake value (SUVmax) and tumour-to-non-tumourous liver uptake (T/NT) ratio were used to quantify 18F-FDG uptake. In vitro 18F-FDG uptake assay of GPC3-expressing HepG2 and non–GPC3-expressing RH7777 cells was used to examine the effect of GPC3 in cellular glucose metabolism. The relationships between GPC3 expression and 18F-FDG uptake, GLUT1 expression, tumour differentiation and other clinical indicators were analysed using Spearman rank correlation, univariate and multiple logistic regression analyses..Results: Positive GPC3 expression was observed in 67.3% of HCC patients, including 75.0% of those with well or moderately differentiated HCC and 36.4% of those with poorly differentiated HCC patients. There was an inverse relationship between GPC3 expression and SUVmax (Spearman correlation coefficient=-0.281, P=0.038) and a positive relationship between GLUT1 expression and SUVmax (Spearman correlation coefficient= 0.681, P 0.05). Further multivariate analysis revealed that only the T/N ratio was significantly correlated with GPC3 expression in patients with HCC (P<0.05). In vitro assay revealed that the uptake of 18F-FDG in GPC3-expressing HepG2 cells was significantly lower than that of non–GPC3-expressing RH7777 cells (t=-20.352, P<0.001). .Conclusions: Present study demonstrated that GPC3 expression is inversely associated with glucose metabolism, suggesting that GPC3 may play a role in regulating glucose metabolism in HCC..