Development of molecularly imprinted polymers for the binding of nitrofurantoin
Development of molecularly imprinted polymers for the binding of nitrofurantoin
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DOI：
10.1016/j.bios.2009.06.003
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发表时间：
2009-09-15
影响因子：
12.6
通讯作者：
Scheller, Frieder W.
中科院分区：
文献类型：
--
作者：
Athikomrattanakul, Umporn;Katterle, Martin;Scheller, Frieder W.
以2，6-二（甲基丙烯酰胺基）吡啶（BMP）为功能单体，羧苯基氨基海因（CPAH）为模板类似物，在极性溶剂中通过光引发聚合制备了呋喃妥因（NFT）分子印迹聚合物（MIPs）。用1H NMR滴定法测定BMP与呋喃妥因或CPAH在DMSO中的复合物的结合常数分别为630 +/- 104和830 +/- 146 M-1。为了研究官能单体的影响，制备了两种聚合物组合物，其分别含有摩尔比为1：1：12（对于MIP 1）和1：4：20（对于MIP 2）的模板、官能单体和交联剂。在呋喃妥因的饱和浓度下的印迹因子，其是结合到MIP和非印迹对照聚合物（NIP）的量的比率，被确定为对于MIP 1为2.47，对于MIP 2为2.49。印迹聚合物的交叉反应性似乎是由与功能单体形成氢键的能力决定的，而分子的形状没有真实的影响。(C)2009 Elsevier B. V.保留所有权利。
Novel molecularly imprinted polymers (MIPs) for the recognition of nitrofurantoin (NFT) were prepared by photoinitiated polymerisation in polar solvent using 2,6-bis(methacrylamido) pyridine (BMP) as the functional monomer and carboxyphenyl aminohydantoin (CPAH) as the analogue of the template. The binding constants of the complex between BMP and nitrofurantoin or CPAH in DMSO were determined with 1H NMR titration to be 630 +/- 104 and 830 +/- 146 M-1, respectively. To study the influence of the functional monomer, two polymer compositions were prepared containing the template, the functional monomer and the crosslinker in the molar ratio 1:1:12 for MIP1 and 1:4:20 for MIP2, respectively. The imprinting factor at saturation concentration of nitrofurantoin, which is the ratio of the amount bound to the MIP and the non-imprinted control polymer (NIP), was determined to be 2.47 for MIP1 and 2.49 for MIP2. The cross reactivity of the imprinted polymers seems to be determined by the ability to form hydrogen bonds to the functional monomer while the shape of the molecule has no real influence. (C) 2009 Elsevier B.V. All rights reserved.