Alterations in temporal/spatial distribution of GFAP- and vimentin-positive astrocytes after spinal cord contusion with the New York University spinal cord injury device
Alterations in temporal/spatial distribution of GFAP- and vimentin-positive astrocytes after spinal cord contusion with the New York University spinal cord injury device
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DOI：
10.1089/neu.1998.15.1015
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发表时间：
1998-12-01
影响因子：
4.2
通讯作者：
Scheff, SW
中科院分区：
文献类型：
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作者：
Baldwin, SA;Broderick, R;Scheff, SW
星形胶质细胞由于各种类型的损伤而变得具有反应性，并上调2种中间丝、胶质细胞酸性蛋白（GFAP）和发育调节蛋白波形蛋白。使用纽约大学损伤装置使年轻雌性Sprague-Dawley大鼠在脊髓T10节段遭受挫伤。在伤后1、2、7、14和30天处死动物，用抗两种中间丝蛋白的抗体评估跨越T7-T13节段的水平脊髓切片。损伤后2天，灰质GFAP阳性星形胶质细胞数量增加，损伤部位邻近节段（近端）的星形胶质细胞比几个节段（远端）的星形胶质细胞反应更强。损伤后30天，星形胶质细胞数量恢复到正常水平。波形蛋白阳性星形胶质细胞在损伤后2天也显示出分级的近端/远端反应，在损伤后30天，近端区域仍然显著高于对照动物，头端/尾部变化也很明显，损伤尾部区域显示出比头端区域显著更高数量的波形蛋白阳性星形胶质细胞，表明脊髓损伤后脊髓损伤尾侧的灰质区可能承受更多的压力。
Astrocytes become reactive as a result of various types of lesions and upregulate 2 intermediate filaments, glial fibrillary acidic protein (GFAP), and the developmentally regulated protein vimentin, Young female Sprague-Dawley rats were subjected to a spinal cord contusion at segment T10 using the New York University injury device. Animals were killed at 1, 2, 7, 14, and 30 days postinjury, Horizontal spinal cord sections spanning segments T7-T13 were assessed with antibodies to both intermediate filament proteins. The number of gray matter GFAP-positive astrocytes increased by 2 days postinjury, with segments adjacent (proximal) to the injury site showing greater responses than areas several segments away (distal), By 30 days following injury, astroglial cell numbers returned to normal levels. Vimentin-positive astrocytes also showed a graded proximal/distal response by 2 days following injury, Proximal regions remained significantly higher at 30 days following injury than control animals, Rostral/caudal changes were also evident, with regions caudal to the injury showing significantly higher numbers of vimentin positive astrocytes than those rostral, indicating that gray matter areas caudal to spinal cord injury may undergo more stress following spinal cord injury.