The complete nucleotide sequence and gene organization of the mitochondrial genome of the bumblebee, Bombus ignitus (Hymenoptera: Apidae)
The complete nucleotide sequence and gene organization of the mitochondrial genome of the bumblebee, Bombus ignitus (Hymenoptera: Apidae)
复制标题
DOI：
10.1016/j.gene.2006.12.031
复制
发表时间：
2007-05-01
期刊：
影响因子：
3.5
通讯作者：
Kim, Iksoo
中科院分区：
文献类型：
--
作者：
Cha, So Young;Yoon, Hyung Joo;Kim, Iksoo
测定了小蜜蜂Bombus ignitus（膜翅目：蜂科）有丝分裂基因组16434 bp的完整核苷酸序列。基因组包含典型的后生动物有丝分裂基因组的碱基组成和密码子使用。B. ignitus有丝分裂基因组的一个不寻常的特征是存在五个tRNA样结构：两个tRNA（Leu(UUR)）样序列和两个tRNA（Ser(AGN)）样序列和一个tRNA（Phe）样序列。这些trna样序列具有适当的折叠结构和反密码子序列，但它们在各自氨基酸转移中的功能尚不确定。在这些序列中，tRNA(Leu(UUR))-like序列和tRNA(Ser(AGN))-like序列似乎位于富含A+ t的区域。这种tRNA（Ser(AGN)）样序列非常不寻常，因为与其他昆虫（包括蜜蜂）的tRNA（Met）相比，它的序列同源性非常高，但它含有反密码子ACT，将其指定为tRNA（Ser(AGN)）。所有的PCG和rrna都保守在昆虫有丝分裂基因组结构中最常见的位置，但trna的位置是高度可变的，为昆虫有丝分裂基因组提供了一种新的排列方式。从整体上看，在迄今确定的所有昆虫有丝分裂基因组序列中，B. ignitus有丝分裂基因组的a +T含量最高（86.9%）。所有的蛋白质编码序列都以一个典型的ATN密码子开始。13个PCGs中有9个具有完整的终止密码子（均为TAA），但其余4个基因以不完整的TA或t终止，所有tRNA都具有mt tRNA的典型三叶草结构，除了tRNA(Ser(AGN))，其东华大学臂形成一个简单的环。B. ignitus trna的所有反密码子与A. mellifera的反密码子完全相同。在富含A+ t的区域，还存在一个高度保守的序列块，这是先前在直翅目和双翅目中描述的。在该区域还发现了可能在mtDNA复制起始中起作用的茎环结构。以Melipona bicolor （Meliponini）、A. mellifera （Apini）和B. ignitus （Bombini）为代表的3个虫族的系统发育分析表明，M . bicolor与B. ignitus亲缘关系最为密切。(c) 2007 Elsevier B.V.版权所有
The complete 16,434-bp nucleotide sequence of the mitogenome of the humble bee, Bombus ignitus (Hymenoptera: Apidae), was determined. The genome contains the base composition and codon usage typical of metazoan mitogenomes. An unusual feature of the B. ignitus mitogenome is the presence of five tRNA-like structures: two each of the tRNA(Leu(UUR))-like and tRNA(Ser(AGN))-like sequences and one tRNA(Phe)-like sequence. These tRNA-like sequences have proper folding structures and anticodon sequences, but their functionality in their respective amino acid transfers remained uncertain. Among these sequences, the tRNA(Leu(UUR))-like sequence and the tRNA(Ser(AGN))-like sequence are seemingly located within the A+T-rich region. This tRNA(Ser(AGN))-like sequence is highly unusual in that its sequence homology is very high compared to the tRNA(Met) of other insects, including Apis mellifera, but it contains the anticodon ACT, which designates it as tRNA(Ser(AGN)). All PCG and rRNAs are conserved in positions observed most frequently in insect mitogenome structures, but the positions of the tRNAs are highly variable, presenting a new arrangement for an insect mitogenome. As a whole, the B. ignitus mitogenome contains the highest A+T content (86.9%) found in any of the complete insects mt sequences determined to date. All protein-coding sequences started with a typical ATN codon. Nine of the 13 PCGs have a complete termination codon (all TAA), but the remaining four genes terminate with the: incomplete TA or T. All tRNAs have the typical clover-leaf structures of mt tRNAs, except for tRNA(Ser(AGN)), in which the DHU arm forms a simple loop. All anticodons of B. ignitus tRNAs are identical to those of A. mellifera. In the A+T-rich region, a highly conserved sequence block that was previously described in Orthoptera and Diptera was also present. The stem-and-loop structures that may play a role in the initiation of mtDNA replication were also found in this region.Phylogenetic analysis among three corbiculate tribes, represented by Melipona bicolor (Meliponini), A. mellifera (Apini), and B. ignitus (Bombini), showed the closest relationship between M bicolor and B. ignitus. (c) 2007 Elsevier B.V. All rights reserved.