Serological differentiation of human papillomavirus types 11, 16 and 18 using recombinant virus-like particles.
Serological differentiation of human papillomavirus types 11, 16 and 18 using recombinant virus-like particles.
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DOI：
10.1099/0022-1317-75-9-2445
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发表时间：
1994-09
期刊：
影响因子：
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通讯作者：
R. Rose;W. Bonnez;Caroline Da Rin;D. McCance;R. Reichman
中科院分区：
文献类型：
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作者：
R. Rose;W. Bonnez;Caroline Da Rin;D. McCance;R. Reichman
人乳头瘤病毒（HPV）11、16和18型的L1主要衣壳蛋白编码序列在杆状病毒系统中表达。从重组感染的草地贪夜蛾Sf 9细胞和无细胞培养上清液中纯化病毒样颗粒（VLP）。用纯化的VLP免疫的兔产生仅与用作免疫原的特定VLP类型反应的抗体。此外，针对感染性HPV-11病毒体产生的兔抗体仅与HPV-11 L1 VLP反应，而不与来自HPV-16或HPV-18的VLP反应。这些结果表明HPV-11、HPV-16和HPV-18病毒粒子在抗原性上彼此不同。这一观察结果应在未来对HPV免疫反应的研究中加以考虑。
The L1 major capsid protein-coding sequences of human papillomavirus (HPV) types 11, 16 and 18 were expressed in the baculovirus system. Virus-like particles (VLPs) were purified from recombinant-infected Spodoptera frugiperda Sf9 cells and cell-free culture supernatants. Rabbits immunized with purified VLPs developed antibodies that reacted only with the specific VLP type used as the immunogen. In addition, rabbit antibodies raised against infectious HPV-11 virions only reacted with HPV-11 L1 VLPs and not with VLPs derived from either HPV-16 or HPV-18. These results suggest that HPV-11, HPV-16 and HPV-18 virions are antigenically distinct from one another. This observation should be considered in future studies of immune responses to HPV.