Analysis of DNA adducts using high-performance separation techniques coupled to electrospray ionization mass spectrometry
Analysis of DNA adducts using high-performance separation techniques coupled to electrospray ionization mass spectrometry
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DOI：
10.1016/s0021-9673(99)00779-7
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发表时间：
1999-09-24
影响因子：
4.1
通讯作者：
Harsch, A
中科院分区：
文献类型：
--
作者：
Andrews, CL;Vouros, P;Harsch, A
致癌物-DNA共价加合物是一类重要的生物标志物，其鉴定和定量是毒理学研究的一个重要目标。由于这些加合物通常在体内样品中以非常低的水平存在，因此灵敏和特异的分析方法对于准确检测、表征和定量是必要的。高性能分离与电喷雾质谱联用（ESI-MS）提供了分析DNA加合物所需的灵敏度和特异性。本文综述了这一领域的研究进展，重点介绍了HPLC-ESI-MS和CE-ESI-MS技术在修饰核苷、核苷酸和寡核苷酸结构分析和定量中的应用。(C)1999 Elsevier Science B. V.保留所有权利。
Identification and quantitation of covalent carcinogen-DNA adducts, an important class of biomarkers, is an integral goal in toxicological research. Since these adducts are commonly present at very low levels in in vivo samples, sensitive and specific analytical methodologies are imperative for accurate detection, characterization and quantitation. High-performance separations coupled to electrospray mass spectrometry (ESI-MS) provide the sensitivity and specificity required for the analysis of DNA adducts. This review provides an overview over the research conducted in this area, focusing on the application of HPLC-ESI-MS and CE-ESI-MS techniques for structural analysis and quantitation of modified nucleosides, nucleotides and oligonucleotides. (C) 1999 Elsevier Science B.V. All rights reserved.