Ethylene Enhances Seed Germination and Seedling Growth Under Salinity by Reducing Oxidative Stress and Promoting Chlorophyll Content via ETR2 Pathway
Ethylene Enhances Seed Germination and Seedling Growth Under Salinity by Reducing Oxidative Stress and Promoting Chlorophyll Content via ETR2 Pathway
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乙烯通过 ETR2 途径减少氧化应激并促进叶绿素含量，从而增强盐度下的种子发芽和幼苗生长
DOI：
10.3389/fpls.2020.01066
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发表时间：
2020-07-16
影响因子：
5.6
通讯作者：
Wuriyanghan, Hada
中科院分区：
文献类型：
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作者：
Wang, Yue;Diao, Pengfei;Wuriyanghan, Hada
紫花苜蓿(Medicago sativaL.)是一种重要的牧草，盐度是影响其产量的主要胁迫因素。在本研究中，我们发现渗透胁迫抑制了紫花苜蓿幼苗的生长，而离子/氧化胁迫则降低了其种子萌发。乙烯处理可以恢复盐胁迫下紫花苜蓿种子的发芽率，而乙烯抑制剂硫代硫酸银则加剧了盐胁迫的影响。ETH减少了丙二醛和H(2)O(2)的积累，提高了POD活性。ETH和ACC通过增加盐胁迫下紫花苜蓿体内的脯氨酸含量来提高紫花苜蓿的耐盐性。相反，STS通过降低POD活性来抑制紫花苜蓿种子萌发。氯化钠处理降低了紫花苜蓿叶片中的叶绿素含量，乙酸乙酯和乙酸乙酯能增加紫花苜蓿叶片中的叶绿素含量，促进幼苗生长。ETH通过降低MsACO和MsERF8基因的表达来促进盐胁迫下紫花苜蓿的生长，而通过上调MsERF11基因来提高其发芽率。沉默紫花苜蓿中一个可能的乙烯受体基因MsETR2，取消了乙烯诱导的盐胁迫耐性。综上所述，我们证明了乙烯以MsETR2依赖的方式提高了紫花苜蓿的耐盐性，并分析了盐胁迫下乙烯对紫花苜蓿种子萌发的调控机制。
Alfalfa (Medicago sativaL.) is an important forage, and salinity is a major stress factor on its yield. In this study, we show that osmotic stress retards alfalfa seedling growth, while ionic/oxidative stress reduces its seed germination. Ethylene treatment can recover the germination rate of alfalfa seeds under salt stress, while ethylene inhibitor silver thiosulfate exacerbates salt effects. ETH reduces the accumulation of MDA and H(2)O(2)and increases POD activity. ETH and ACC improve the salt tolerance of alfalfa by increasing proline content under salt stress. In contrast, STS inhibits alfalfa seed germination by reducing POD activity. NaCl treatment reduces chlorophyll content in alfalfa leaves, while ETH and ACC can increase the chlorophyll content and promote seedling growth. ETH promotes the growth of alfalfa in saline condition by reducing the expression ofMsACOandMsERF8genes, while increases its germination rate by upregulatingMsERF11gene. Silencing ofMsETR2, a putative ethylene receptor gene in alfalfa, abolishes ethylene triggered tolerance to salt stress. In summary, we show that ethylene improves salt tolerance in alfalfavia MsETR2dependent manner, and we also analyze the regulatory mechanism of ethylene during germination of alfalfa seeds under salt stress.