Induction of tetraploidy using high temperature exposure during the first zygote division in Populus adenopoda Maxim
Induction of tetraploidy using high temperature exposure during the first zygote division in Populus adenopoda Maxim
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DOI：
10.1007/s10725-013-9859-7
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发表时间：
2014-04-01
影响因子：
4.2
通讯作者：
Chen, Yong
中科院分区：
文献类型：
--
作者：
Lu, Min;Zhang, Pingdong;Chen, Yong
三倍体育种是杨树改良的一条有效途径。通过二倍体与四倍体杂交是获得三倍体杨树的理想途径。本研究试图通过高温处理抑制四倍体杨的第一次受精卵分裂，提高四倍体的生产效率。分析了受精卵分裂时期与卵巢棉状纤维发育变化的关系，为准确选择细胞学分期提供指导。在3,582个后代中，通过流式细胞术分析和染色体数目计数证实了32个四倍体。四倍体诱导的最高效率为14.12%。二倍体和四倍体之间气孔平均大小和密度存在显着差异。四倍体植物表现出改变的形态特征，如更厚的叶子，改变的叶子形态，和发育不良的生长。结果表明，在合子第一次分裂时进行辐射处理是诱导杨树四倍体的理想方法。
Triploid breeding has proven to be a valuable approach in Populus improvement. An ideal way to achieve triploid Populus is to cross diploids with tetraploids. In this study, we attempted to induce tetraploids of Populus adenopoda by inhibiting the first zygote division with high temperature exposure and increase the efficiency of tetraploid production. The relationship between zygote division stages and developmental changes of the cotton-like fiber in ovaries were analyzed, helping to guide the treatment towards the most accurate cytological stage. Among the 3,582 offspring, 32 teraploids were confirmed by both flow cytometric analysis and chromosome number counting. The highest efficacy of teraploid induction was 14.12 %. Significant differences between diploids and tetraploids were observed between the mean sizes and densities of leaf stoma. The tetraploid plants showed modified morphological characteristics, such as thicker leaves, modified leaf morphology, and stunted growth. Our finding showed that exposure during the first zygote division is an ideal method for teraploid induction in Populus.