Is Alba an RNase P subunit?
Is Alba an RNase P subunit?
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DOI：
10.4161/rna.4.3.5347
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发表时间：
2007-07-01
期刊：
影响因子：
4.1
通讯作者：
Brown, James W.
中科院分区：
文献类型：
--
作者：
Ellis, J. Chris;Barnes, Jeffrey;Brown, James W.
Alba是古细菌中一种成熟的染色质蛋白，也是古细菌核糖核酸酶P全酶的一个亚基，这是基于对该蛋白在人类中的同源物与核糖核酸酶P活性相关的观察。使用相同的生物化学方法，我们以前使用的显示，其他四个蛋白质同源真核RNase P蛋白是真正的RNase P亚基在大肠杆菌，我们不能检测到任何关联的阿尔巴同源物在甲烷热自养菌（Mth 1483p）与RNase P全酶。此外，Mth 1483p的存在并不增强从重组亚基重构的RNase P全酶的活性。总之，我们没有发现Alba是RNase P亚基的证据。
It has been suggested that Alba, a well-established chromatin protein in Archaea, is also a subunit of the archaeal RNase P holoenzyme, based on the observation that the homolog of this protein in humans has been shown to be associated with RNase P activity. Using the same biochemical methods we used previously to show that four other proteins homologous to eukaryotic RNase P proteins are bona fide RNase P subunits in Archaea, we could not detect any association of the Alba homolog in Methanothermobacter thermoautotrophicus (Mth 1483p) with the RNase P holoenzyme. In addition, the presence of Mth 1483p did not enhance the activity of RNase P holoenzyme reconstituted from recombinant subunits. In conclusion, we find no evidence that Alba is an RNase P subunit.