Incorporation of electrospun nanofibrous PVDF membranes into a microfluidic chip assembled by PDMS and scotch tape for immunoassays
Incorporation of electrospun nanofibrous PVDF membranes into a microfluidic chip assembled by PDMS and scotch tape for immunoassays
复制标题
将电纺纳米纤维 PVDF 膜纳入由 PDMS 和透明胶带组装的微流控芯片中，用于免疫分析
DOI：
10.1002/elps.200900128
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发表时间：
2009-09-01
期刊：
影响因子：
2.9
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
--
作者：
Liu, Yingyi;Yang, Dayong;Jiang, Xingyu
本文介绍了一种用于PVDF电纺纳米纤维膜的多种免疫分析的微流控芯片。这种微芯片能够通过跨越平行的微通道阵列，在一次实验中检测多对蛋白质-蛋白质相互作用。该芯片使用具有高比表面积的ESPVDF膜作为底物吸附蛋白质用于固定化免疫分析，从而提高蛋白质的吸附水平可以降低免疫分析中的LOD。我们采用了一种简单有效的透明胶带组装芯片的方法，将ESPVDF膜整合到微芯片中。我们相信，本工作将促进基于抗原-抗体识别的疾病现场诊断方法的发展，并提高未来需要以相对较低的成本识别数十到数百种蛋白质-蛋白质相互作用的某些实验的效率。
This paper demonstrates a microfluidic chip for multiple immunoassays on electrospun (ES) nanofibrous membranes made of PVDF. This microchip enables the detection of multiple pairs of protein-protein interactions in one experiment by crossing parallel arrays of microchannels. This chip uses ESPVDF membrane with high specific surface area as the substrate to adsorb protein for immobilized immunoassays so that increased levels of protein adsorption may lower the LOD in immunoassays. We employ a simple and effective method for chip assembly by scotch tape to incorporate the ESPVDF membrane into the microchip. We believe that the present work will facilitate the development of methods for the on-site diagnosis of diseases whose detection is based on antigen-antibody recognition, and improve the efficiencies of certain experiments that need to identify tens to hundreds of protein-protein interactions with relatively low cost in the future.