Seroepidemiology of Entamoeba histolytica in a slum in northeastern Brazil
Seroepidemiology of Entamoeba histolytica in a slum in northeastern Brazil
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DOI：
10.4269/ajtmh.1996.55.693
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发表时间：
1996-12-01
影响因子：
3.3
通讯作者：
Mann, BJ
中科院分区：
文献类型：
--
作者：
Braga, LL;Lima, AAM;Mann, BJ
在巴西东北部，人类致病性寄生虫溶组织内阿米巴的感染尚未得到很好的表征。在这项研究中，E。使用酶联免疫吸附试验（ELISA）检测针对大肠杆菌半乳糖/N-乙酰基-D-半乳糖胺（Gal/GalNAc）可抑制的粘附凝集素的抗体，检测巴西东北部贫民窟的溶组织菌感染。溶组织剂对来自总共335个个体的血清检查抗Gal/GalNAc凝集素抗体。总体血清阳性率为24.7%; 29.4%的女性和19.4%的男性为阳性。在不同年龄组中，6-14岁年龄组的阳性率最高，为40%。血清阳性也有家族聚集性。为了检查寄生虫的定植，对155人的粪便样本进行了显微镜检查，以确定寄生虫的存在。155份粪便中有14份（9.0%）被鉴定为含有E。溶组织性或非致病性E.差异这14例阳性粪便用ELISA分析，检测Gal/GalNAc凝集素抗原，并可区分E. histolytica和E.差异粪便中有4份（29%）为E. 10株为溶组织E.阳性。虽然显微镜下的总定植率仅为9%，其中三分之一被鉴定为E。在溶组织性大肠杆菌感染中，高达40%的年龄较大的儿童出现了感染致病性大肠杆菌的血清学证据。溶组织感染研究结果表明，巴西东北部的这一群落是E.感染率与其他发展中国家相似。
Infection with the human pathogenic parasite Entamoeba histolytica has not been well-characterized in northeastern Brazil. In this study, the prevalence of E. histolytica infection in a slum in northeastern Brazil was assayed using an enzyme-linked immunosorbent assay (ELISA) for antibodies against the galactose/N-acetyI-D-galactosamine (Gal/GalNAc)-inhibitable adherence lectin of E. histolytica. Sera from a total of 335 individuals were examined for anti-Gal/GalNAc lectin antibodies. The overall seropositivity was 24.7%; 29.4% of females and 19.4% of males were positive. Among different age groups there was a peak of 40% positivity in the 6-14-year-old age group. There was also familial clustering of seropositivity. To examine colonization, stool samples from 155 people were examined microscopically for the presence of the parasite. Fourteen of 155 stools (9.0%) were identified as containing E. histolytica or nonpathogenic E. dispar. These 14 positive stools were analyzed with an ELISA that detects Gal/GalNAc lectin antigen and can distinguish between E. histolytica and E. dispar. Four stools (29%) were positive for E. histolytica and the remaining 10 were identified as E. dispar-positive. Although the overall colonization rate by microscopy was only 9%, with a third identified as E. histolytica, up to 40% of older children develop serologic evidence of having experienced pathogenic E. histolytica infection. The results of this study demonstrate that this community in northeastern Brazil is highly endemic for E. histolytica with infection rates similar to other developing nations.