Gastrointestinal tract distribution of Salmonella enteritidis in orally infected mice with a species-specific fluorescent quantitative polymerase chain reaction
Gastrointestinal tract distribution of Salmonella enteritidis in orally infected mice with a species-specific fluorescent quantitative polymerase chain reaction
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DOI：
10.3748/wjg.13.6568
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发表时间：
2007-12-28
影响因子：
4.3
通讯作者：
Cao, Ping
中科院分区：
文献类型：
--
作者：
Deng, Shu-Xuan;Cheng, An-Chun;Cao, Ping
目的：识别和了解肠炎沙门氏菌（S.enteritidis）在胃肠道中的规律分布模式和主要渗透位点。方法：根据GenBank中肠炎沙门氏菌种属特异性DNA序列，建立种属特异性实时荧光定量聚合酶链反应（FQ-PCR）用于检测肠炎沙门氏菌。我们使用该方法检测口腔感染后小鼠胃肠道（包括十二指肠、空肠、回肠、盲肠、结肠、直肠、食道和胃）中 5 种肠炎沙门氏菌的基因组 DNA。 结果：肠炎沙门氏菌在胃肠道的所有部分中均被检测到。接种后8小时空肠和回肠呈阳性，接种后18小时最后出现阳性结果的器官是胃。各组织中肠炎沙门氏菌DNA拷贝数在接种后24-36 h达到峰值，其中空肠、回肠和盲肠含有高浓度的肠炎沙门氏菌，而十二指肠、结肠、直肠、胃和食管的浓度较低。接种后2天肠炎沙门氏菌开始减少并消失，但接种后5天仍存在于空肠、回肠和盲肠中，且未引起明显症状。接种后5 d，盲肠中肠炎沙门氏菌数量显着高于其他区域（P < 0.01），这似乎反映了其作为肠炎沙门氏菌储存库的功能。结论：该结果为阐明肠炎沙门氏菌在胃肠道中的致病机制提供了重要数据，并表明空肠、回肠和盲肠是正常小鼠口腔感染后的主要侵袭部位。这项研究将有助于进一步了解肠炎沙门氏菌的作用机制。 (C) 2007 WJG。版权所有。
AIM: To identify and understand the regular distribution pattern and primary penetration site for Salmonella enteritidis (S. enteritidis) in the gastrointestinal tract.METHODS: Based on the species-specific DNA sequence of S. enteritidis from GenBank, a species-specific realtime, fluorescence-based quantitative polymerase chain reaction (FQ-PCR) was developed for the detection of S. enteritidis. We used this assay to detect genomic DNA of 5, enteritidis in the gastrointestinal tract, including duodenum, jejunum, ileum, cecum, colon, rectum, esophagus and stomach, from mice after oral infection.RESULTS: S. enteritidis was consistently detected in all segments of the gastrointestinal tract. The jejunum and ileum were positive at 8 h post inoculation, and the final organ to show a positive result was the stomach at 18 h post inoculation. The copy number of S. enteritidis DNA in each tissue reached a peak at 24-36 h post inoculation, with the jejunum, ileum and cecum containing high concentrations of S. enteritidis, whereas the duodenum, colon, rectum, stomach and esophagus had low concentrations. S. enteritidis began to decrease and vanished at 2 d post inoculation, but it was still present up to 5 d post inoculation in the jejunum, ileum and cecum, without causing apparent symptoms. By 5 d post inoculation, the cecum had significantly higher numbers of S. enteritidis than any of the other areas (P < 0.01), and this appeared to reflect its function as a repository for S. enteritidis.CONCLUSION: The results provided significant data for clarifying the pathogenic mechanism of S. enteritidis in the gastrointestinal tract, and showed that the jejunum, ileum and cecum are the primary sites of invasion in normal mice after oral infection. This study will help to further understanding of the mechanisms of action of S. enteritidis. (C) 2007 WJG. All rights reserved.