Validation of radioimmunoassays for two salmon gonadotropins (GTH I and GTH II) and their plasma concentrations throughout the reproductive cycle in male and female rainbow trout (Oncorhynchus mykiss)
Validation of radioimmunoassays for two salmon gonadotropins (GTH I and GTH II) and their plasma concentrations throughout the reproductive cycle in male and female rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1095/biolreprod54.6.1375
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发表时间：
1996-06-01
影响因子：
3.6
通讯作者：
Tyler, CR
中科院分区：
文献类型：
--
作者：
Prat, F;Sumpter, JP;Tyler, CR
RIA是为两种鲑鱼促性腺激素(GTH I和GM II)开发的，用于在虹鲑鱼的整个生命过程中测量血浆GTH。放射免疫法检测GTH-I特异性强，灵敏度高(与GTH-I交叉反应率为0.001%，平均灵敏度为0.26+/-0.02 ng/ml)。放射免疫法检测GTH-I的特异性和敏感性均低于GTH-II(与GTH-II的交叉反应率为9.7%，平均灵敏度为2.34+/-0.23 ng/ml)。无论男性还是女性，GTH-II的水平在生殖周期的大部分时间内都是检测不到的(-0.3 ng/ml)，直到受精/排卵前不久才开始升高。血浆GTH-II浓度在受精/排卵时最高。无论男女，GTH I和GTH II的血浆浓度不同，女性在卵黄发生早期血浆GTH I浓度升高。然后在排卵前不久下降到基础水平，最后在排卵时再次升高。在男性中，血浆GTH I在受精前一年和之后的睾丸生长的最后阶段再次升高。这些结果支持GTH-I介导性腺生长，而GTH-II调节成熟和排卵/受精的最后阶段的观点。在虹鲑鱼中，GTH-I和GTH-II的血浆谱分别模拟高等脊椎动物排卵周期中血浆FSH和LH的周期。
RIAs were developed for the two salmon gonadotropins (GTH I and GM II) and used to measure plasma GTH throughout the life of the rainbow trout, Oncorhynchus mykiss. The RIA for GTH II was specific and sensitive (< 0.001% cross-reaction with GTH I, mean sensitivity = 0.26 +/- 0.02 ng/ml). The RIA for GTH I was less specific and less sensitive than the GTH II RIA (9.7% cross-reaction with GTH II, mean sensitivity = 2.34 +/- 0.23 ng/ml).In both males and females, the levels of GTH II remained undetectable (< 0.3 ng/ml) throughout most of the reproductive cycle, until shortly preceding spermiation/ovulation, when they began to rise. Concentrations of plasma GTH II were maximal at spermiation/ovulation. In both sexes, plasma profiles of GTH I differed from those of GTH II. The plasma GTH I concentration in females was elevated during early vitellogenesis. It then fell to a basal level shortly before ovulation and finally was elevated again at ovulation. In males, increases in plasma GTH I were seen a year before spermiation and again later, during the final stages of testicular growth. These results support the contention that GTH I mediates gonadal growth, whereas GTH II regulates the final stages of maturation and ovulation/spermiation.In rainbow trout, plasma profiles of GTH I and GTH II mimic the cycles of plasma FSH and LH, respectively, in the ovulatory cycle of higher vertebrates.