ZOONOTIC POTENTIAL OF GIARDIASIS IN DOMESTIC RUMINANTS
ZOONOTIC POTENTIAL OF GIARDIASIS IN DOMESTIC RUMINANTS
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DOI：
10.1093/infdis/162.1.231
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发表时间：
1990-07-01
影响因子：
6.4
通讯作者：
OLSON, ME
中科院分区：
文献类型：
--
作者：
BURET, A;DENHOLLANDER, N;OLSON, ME
本研究旨在评估贾第虫病在国内反刍动物中的流行情况和人畜共患的可能性。绵羊和牛的感染率分别为17.7%和10.4%，羔羊和小牛的感染率显著高于绵羊和牛（分别为35.6%和27.7%）。自然感染的羔羊在几个月内间歇性地释放囊肿。绵羊贾第鞭毛虫滋养体具有典型的爪锤状中间体，在TYI-S-33培养基中成功培养，细胞质、细胞骨架和膜组分的蛋白质谱与人分离物相似。免疫印迹法显示，感染羊的血清可识别人贾第鞭毛虫，贾第鞭毛虫病患者的血清可识别羊的贾第鞭毛虫，两种情况下的识别均涉及分子量相似的抗原蛋白。绵羊感染的囊肿输出和临床症状与人类疾病相似，感染人类和反刍动物的生物体在形态和抗原上相似。据推测，家庭反刍动物可能是人类感染的宿主，反之亦然，因此将贾第虫病归类为动物人畜共患病。
This study was conducted to assess the prevalence and zoonotic potential of giardiasis in domestic ruminants. Prevalence of infection was 17.7% in sheep and 10.4% in cattle and was significantly higher in lambs and calves (35.6% and 27.7%, respectively). Naturally infected lambs released cysts intermittently for months. Giardia trophozoites from sheep had typical claw hammer-shaped median bodies and were successfully cultured in TYI-S-33 medium, and cytosolic, cytoskeletal, and membrane fractions exhibited protein profiles similar to human isolates (WB). Immunoblotting showed that sera from infected sheep recognized human Giardia, sera from patients with giardiasis recognized Giardia from sheep, and in both cases recognition involved antigenic proteins of similar molecular weight. Cyst output and clinical signs in ovine infection resemble human disease and the organisms infecting humans and ruminants are morphologically and antigenically similar. It is postulated that domestic ruminants may be a reservoir for human infection and vice versa, thus classifying giardiasis as a zooanthoponotic disease.