Simultaneously disrupting AtPrx2, AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem
Simultaneously disrupting AtPrx2, AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem
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DOI：
10.1111/jipb.12334
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发表时间：
2015-04-01
影响因子：
11.4
通讯作者：
Tsutsumi, Yuji
中科院分区：
文献类型：
--
作者：
Shigeto, Jun;Itoh, Yoshitaka;Tsutsumi, Yuji
植物III类血红素过氧化物酶催化木质素聚合。先前的报道表明，至少有三种拟南芥过氧化物酶，AtPrx 2，AtPrx 25和AtPrx 71，参与使用T-DNA插入突变体，atprx 2，atprx 25和atprx 71的茎木质化。在这里，我们产生了三个双突变体，atprx 2/atprx 25，atprx 2/atprx 71，和atprx 25/atprx 71，并研究了同时缺乏这些过氧化物酶对木质素和植物生长的影响。茎组织分析使用乙酰溴的方法和衍生化，然后还原裂解揭示了改善木质素的特性，如降低木质素含量和增加芳基甘油-芳基（-O-4）连接类型，特别是-O-4连接的双辛基单位，在木质素，支持这些基因在木质素聚合的作用。此外，没有一个双突变体表现出严重的生长缺陷，如植株矮小，矮化，或不育，和他们的茎细胞壁降解能力提高。本研究将有助于木质素生物工程的进展，以提高木质纤维素生物量。
Plant class III heme peroxidases catalyze lignin polymerization. Previous reports have shown that at least three Arabidopsis thaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, are involved in stem lignification using T-DNA insertion mutants, atprx2, atprx25, and atprx71. Here, we generated three double mutants, atprx2/atprx25, atprx2/atprx71, and atprx25/atprx71, and investigated the impact of the simultaneous deficiency of these peroxidases on lignins and plant growth. Stem tissue analysis using the acetyl bromide method and derivatization followed by reductive cleavage revealed improved lignin characteristics, such as lowered lignin content and increased arylglycerol--aryl (-O-4) linkage type, especially -O-4 linked syringyl units, in lignin, supporting the roles of these genes in lignin polymerization. In addition, none of the double mutants exhibited severe growth defects, such as shorter plant stature, dwarfing, or sterility, and their stems had improved cell wall degradability. This study will contribute to progress in lignin bioengineering to improve lignocellulosic biomass.