A Chemical Probe that Labels Human Pluripotent Stem Cells
A Chemical Probe that Labels Human Pluripotent Stem Cells
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DOI：
10.1016/j.celrep.2014.02.006
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发表时间：
2014-03-01
期刊：
影响因子：
8.8
通讯作者：
Uesugi, Motonari
中科院分区：
文献类型：
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作者：
Hirata, Nao;Nakagawa, Masato;Uesugi, Motonari
一种特异于人类多能干细胞的小分子荧光探针将为基础细胞生物学研究和干细胞治疗提供有用的工具。用人诱导多能干细胞（iPSC）筛选荧光化学文库，随后评价命中分子，鉴定出选择性标记人多能干细胞的荧光化合物（京都探针1 [KP-1]）。我们的分析表明，选择性结果主要来自ABC转运蛋白在人多能干细胞中的不同表达模式和KP-1的转运蛋白选择性。ABCB 1（MDR 1）和ABCG 2（BCRP）的表达，这两者都导致KP-1的外排，在人多能干细胞中受到抑制。虽然KP-1，像其他多能标记物一样，对多能干细胞不是绝对特异性的，但所鉴定的化学探针可以与其他试剂结合使用。
A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.