Detection of cytomegalovirus in cerebrospinal fluid autopsy specimens from AIDS patients.
Detection of cytomegalovirus in cerebrospinal fluid autopsy specimens from AIDS patients.
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艾滋病患者脑脊液尸检标本中巨细胞病毒的检测。
DOI：
10.1093/infdis/169.3.623
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Wiley,CA
中科院分区：
文献类型：
--
作者：
Achim,CL;Nagra,RM;Wang,R;Nelson,JA;Wiley,CA
巨细胞病毒(CMV)是感染艾滋病患者的一种常见的条件致病菌。应用聚合酶链式反应和抗原捕获酶联免疫吸附试验检测了40例艾滋病患者脑脊液标本中的巨细胞病毒。用聚合酶链式反应在70%的样本中检测到CMV DNA。在21例系统性CMV感染患者中，57%的患者患有CMV脑炎，而81%的患者脑脊液中存在病毒。在24例无CMV脑炎组织学证据的患者中，58%的脑脊液标本经聚合酶链式反应检测出CMV DNA。这些结果表明，脑脊液聚合酶链式反应能灵敏地检测到系统性巨细胞病毒感染，但对艾滋病患者尸检标本中的脑部感染没有特异性。
Cytomegalovirus (CMV) is a common opportunistic pathogen infecting AIDS patients. Polymerase chain reaction (PCR) and antigen capture ELISA were used to detect CMV in 40 cerebrospinal fluid autopsy specimens from patients with AIDS. CMV DNA was detected by PCR in 70% of samples. Of the 21 samples from patients with systemic CMV infection, 57% had CMV encephalitis, while 81% had virus in cerebrospinal fluid detectable by PCR. Of the 24 samples from patients with no histologic evidence of CMV encephalitis, 58% had CMV DNA in cerebrospinal fluid detected by PCR. These results suggest that PCR of cerebrospinal fluid sensitively detects systemic CMV infection but is not specific for brain infection in autopsy specimens of AIDS patients.