The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of DSS used to induce it.
The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of DSS used to induce it.
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DOI：
10.1186/1472-6890-1-3
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发表时间：
2001
影响因子：
--
通讯作者：
Doe WF
中科院分区：
文献类型：
--
作者：
Stevceva L;Pavli P;Husband AJ;Doe WF
实验性结肠炎的特征类似于炎症性肠病(IBD)，最初已被描述。对炎性细胞的详细分析还没有描述。因此，在这项研究中，我们描述了结肠炎急性期的细胞特征，并将这些发现与已知的人类IBD进行了比较。给BALB/C和C57BL6小鼠灌胃2.5%和5%DSS 8天，建立小鼠结肠炎模型。免疫组织化学F4/80和ER-MP20标记巨噬细胞，TIB 120标记MHC Class II，抗CD4和抗CD8抗体标记细胞。他们是通过使用一种使用视频图像分析的新方法进行列举的。免疫荧光染色检测免疫球蛋白生成细胞，共聚焦显微镜计数。葡聚糖硫酸钠(DSS)诱导的结肠炎急性期的炎性浸润物主要由巨噬细胞、中性粒细胞和嗜酸性粒细胞组成。中性粒细胞数量增加，隐窝脓肿也可见。巨噬细胞数量的增加是由于最近从外周循环中招募的单核细胞。T细胞的数量或分布似乎没有任何变化。炎症对免疫球蛋白产生细胞的影响最大，其中免疫球蛋白产生细胞受影响最大。对免疫球蛋白产生细胞的影响取决于用于诱导结肠炎的DSS的百分比。一般来说，2.5%的DSS会导致这些细胞的增加，5%的DSS会导致这些细胞的耗尽。
Experimental colitis with features similar to inflammatory bowel disease (IBD) has initially been described. A detailed analysis of inflammatory cells has not yet been described. Therefore in this study we characterized the cells involved in the acute phase of the colitis and compared those findings to what is known about human IBD. Colitis was induced in BALB/C and C57Bl6 mice by ingestion of 2.5% and 5% DSS in the drinking water for 8 days. Cells were labelled by immunohistochemical staining with F4/80 and ER-MP20 for macrophages, TIB 120 for MHC Class II presentation, and anti-CD4 and anti-CD8 antibodies. They were enumerated by using a novel method that employs video image analysis. Immunoglobulin-producing cells were enumerated by immunofluorescent staining for IgA, IgG and IgM and counting by using confocal microscopy. Inflammatory infiltrate in the acute phase of the dextran sulphate sodium (DSS) -induced colitis consists predominantly of macrophages, neutrophils and eosinophils. Neutrophils increase in numbers and crypt abscesses were also seen. Increased macrophage numbers were due to recently recruited monocytes from the peripheral circulation. It does not appear that there are any changes in T cell numbers or distribution. The inflammation induced changes in immunoglobulin-producing cells with IgA-producing cells affected the most. The effect on Ig-producing cells depends on the percentage of DSS used to induce colitis. In general, 2.5% DSS induces an increase and 5% DSS a depletion of these cells.