ROLE OF ENDOGENOUS PLATELET-DERIVED GROWTH-FACTOR IN ARTERIAL SMOOTH-MUSCLE CELL-MIGRATION AFTER BALLOON CATHETER INJURY
ROLE OF ENDOGENOUS PLATELET-DERIVED GROWTH-FACTOR IN ARTERIAL SMOOTH-MUSCLE CELL-MIGRATION AFTER BALLOON CATHETER INJURY
复制标题
DOI：
10.1161/01.atv.13.8.1218
复制
发表时间：
1993-08-01
期刊：
影响因子：
--
通讯作者：
REIDY, MA
中科院分区：
文献类型：
--
作者：
JACKSON, CL;RAINES, EW;REIDY, MA
大鼠颈动脉去内皮化损伤后内膜增厚的过程依赖于平滑肌细胞从中膜的迁移。最近的报告表明，血小板衍生生长因子可能是损伤后迁移的重要介质。我们通过直接测定血小板耗尽的动物受伤动脉中的平滑肌细胞迁移以及在施用阻断血小板衍生生长因子的抗体后解决了这个问题。由于据报道纤溶酶原激活剂合成与平滑肌细胞迁移之间存在关联，因此我们测定了动脉损伤后纤溶酶原激活剂的活性水平，并评估了纤溶酶抑制剂对迁移的影响。数据表明，血小板在动脉损伤部位释放的血小板衍生生长因子是平滑肌细胞迁移的内源性介质。由组织型纤溶酶原激活剂催化的纤溶酶生成对于迁移是必需的；血小板衍生生长因子发挥作用的一种方式是刺激平滑肌细胞合成组织型纤溶酶原激活剂。
The process of intimal thickening after de-endothelializing injury to the rat carotid artery is dependent on the migration of smooth muscle cells from the media. Recent reports have suggested that platelet-derived growth factor may be an important mediator of migration after injury. We have addressed this issue by directly determining smooth muscle cell migration in injured arteries of animals depleted of platelets and after administration of an antibody that blocks platelet-derived growth factor. Because there is a reported association between plasminogen activator synthesis and smooth muscle cell migration, we assayed the activity levels of plasminogen activators after arterial injury and also assessed the effect of a plasmin inhibitor on migration. The data suggest that platelet-derived growth factor, released by platelets at sites of arterial injury, is an endogenous mediator of smooth muscle cell migration; that plasmin generation, catalyzed by tissue-type plasminogen activator, is necessary for migration; and that one way in which platelet-derived growth factor may act is by stimulation of the synthesis of tissue-type plasminogen activator by smooth muscle cells.