Near-Infrared Mesoporous Silica Nanoparticles for Optical Imaging: Characterization and In Vivo Biodistribution
Near-Infrared Mesoporous Silica Nanoparticles for Optical Imaging: Characterization and In Vivo Biodistribution
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DOI：
10.1002/adfm.200800753
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发表时间：
2009-01-23
影响因子：
19
通讯作者：
Lo, Leu-Wei
中科院分区：
文献类型：
--
作者：
Lee, Chia-Hung;Cheng, Shih-Hsun;Lo, Leu-Wei
介绍了一种适用于在体高效光学成像的近红外（NIR）介孔二氧化硅纳米粒子（MSN）的表征方法。通过将三甲基铵基团引入到MSN的骨架中，合成了平均粒径为50-100 nm的三甲基铵改性MSN（MSN-TA）。通过静电吸引进一步与吲哚菁绿色（ICG）吸附，使MSN-TA-ICG成为用于体内光学成像的有效近红外造影剂。MSN-TA-ICG对pH的稳定性研究表明，MSN-TA-ICG在酸性至生理pH范围内稳定，在麻醉大鼠体内的生物分布表明，MSN-TA-ICG在肝脏中有较强而稳定的荧光。硅的透射电子显微镜（TEM）成像和元素分析进一步证实了MSN-TA-ICG在主要器官中的物理和定量驻留。这是第一次报道MSN功能化与NIR-ICG能够在体内的光学成像。
The characterization of near-infrared (NIR) mesoporous silica nanoparticles (MSN) suitable for in vivo optical imaging with high efficiency is presented. Trimethylammonium groups modified MSN (MSN-TA) with the average size of 50-100 nm was synthesized with incorporation of the TA groups into the framework of MSN. It was further adsorbed with indocyanine green (ICG) by electrostatic attraction to render MSN-TA-ICG as an efficient NIR contrast agent for in vivo optical imaging. The studies in stability of MSN-TA-ICG against pH Indicated the bonding is stable over the range from acidic to physiological pH. The in vivo biodistribution of MSN-TA-ICG in anesthetized rat demonstrated a rather strong and stable fluorescence of MSN-TA-ICG that prominent in the organ of liver. Transmission electron microscopy (TEM) imaging and elemental analysis of silicon further manifested the physical and quantitative residences of MSN-TA-ICG in major organs. This is the first report of MSN functionalized with NIR-ICG capable of optical imaging in vivo.