The promotion of secondary structures in single-stranded DNA by drugs that bind to duplex DNA: an atomic force microscopy study
The promotion of secondary structures in single-stranded DNA by drugs that bind to duplex DNA: an atomic force microscopy study
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DOI：
10.1088/0957-4484/19/38/384016
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发表时间：
2008-09-24
期刊：
影响因子：
3.5
通讯作者：
Dietler, Giovanni
中科院分区：
文献类型：
--
作者：
Adamcik, Jozef;Valle, Francesco;Dietler, Giovanni
我们用原子力显微镜(AFM)研究了单链DNA(SsDNA)在具有插层结合模式(如柔红霉素、放线菌素D和氯喹)或少量沟槽结合模式(如netropsin和berenil)的已知药物存在下的行为。在极低盐条件下，单链DNA分子采用无二级结构的非结构构象。我们观察到，在这些条件下，添加与双链DNA(DsDNA)结合的药物可以促进单链DNA二级结构的形成。此外，随着药物浓度的增加，这些发夹的延伸性和热稳定性也被观察到。
We study the behavior of single-stranded DNA (ssDNA) in the presence of well-known drugs with either an intercalating binding mode, such as daunorubicin, actinomycin D, and chloroquine, or a minor groove binding mode, such as netropsin and berenil, by atomic force microscopy (AFM). At very low salt conditions, ssDNA molecules adopt an unstructured conformation without secondary structures. We observe that under these conditions additions of drugs that bind to double-stranded DNA ( dsDNA) promote the formation of secondary structures in ssDNA. Furthermore, with an increase of concentration of the drugs, the extension as well as the thermal stabilization of these hairpins was observed.