Preparation of Peptide-Functionalized Gold Nanoparticles Using One Pot EDC/Sulfo-NHS Coupling
Preparation of Peptide-Functionalized Gold Nanoparticles Using One Pot EDC/Sulfo-NHS Coupling
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DOI：
10.1021/la2022177
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发表时间：
2011-08-16
期刊：
影响因子：
3.9
通讯作者：
Kanaras, Antonios G.
中科院分区：
文献类型：
--
作者：
Bartczak, Dorota;Kanaras, Antonios G.
尽管双酰亚胺和琥珀酰亚胺广泛用于形成酰胺键（即，在氨基酸偶联中），它们在肽与水溶性羧基封端的胶体金纳米颗粒偶联中的应用仍然具有挑战性。在这篇文章中，我们提出了一个优化的研究成功耦合的KPQPRPLS肽的球形和棒状胶体金纳米粒子。我们表明，浓度，反应时间和化学环境都是实现强大的，肽包被的胶体纳米粒子的形成的关键。琼脂糖凝胶电泳用于缀合物的表征。
Although carbodiimides and succinimides are broadly employed for the formation of amide bonds (i.e., in amino acid coupling), their use in the coupling of peptides to water-soluble carboxylic-terminated colloidal gold nanoparticles remains challenging. In this article, we present an optimization study for the successful coupling of the KPQPRPLS peptide to spherical and rodlike colloidal gold nanoparticles. We show that the concentration, reaction time, and chemical environment are all critical to achieving the formation of robust, peptide-coated colloidal nanoparticles. Agarose gel electrophoresis was used for the characterization of conjugates.