Endometrial glands are required for preimplantation conceptus elongation and survival
Endometrial glands are required for preimplantation conceptus elongation and survival
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DOI：
10.1095/biolreprod64.6.1608
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发表时间：
2001-06-01
影响因子：
3.6
通讯作者：
Spencer, TE
中科院分区：
文献类型：
--
作者：
Gray, CA;Taylor, KM;Spencer, TE
子宫内膜腺分泌假想支持胚胎生长和发育的分子。在绵羊中，子宫内膜腺的形态发生在出生后，并可以通过新生儿孕酮的暴露而表观遗传地消融。由此产生的稳定的成人子宫腺敲除(UGKO)表型在这里被用来检验子宫内膜腺是成功怀孕所必需的假说。成熟的UGKO母羊被反复饲养到能生育的公羊，但在第25天的超声波检查中没有发现怀孕。然后将来自正常超排母羊的7日龄囊胚同步移植到7日龄对照母羊或UGKO母羊。在配种后25-65天的超声检查显示，对照组母羊妊娠成功，而UGKO母羊没有。为了研究早期子宫-胚胎的相互作用，4只对照母羊和8只UGKO母羊被培育成能生育的公羊。在第14天，他们的子宫被冲了出来。每只对照母羊的子宫包含两个正常长度的丝状胚胎。四只UGKO母羊的子宫中没有胚胎。三只UGKO母羊的子宫只有一个严重生长迟缓的管状胚胎，而其余的母羊只有一个丝状胚胎。这些子宫的组织学分析表明，子宫内膜腺密度与胚胎存活和发育状态有直接关系。第14天没有子宫内膜腺的UGKO子宫不支持正常的胚胎发育，也不包含任何胚胎或生长迟缓的管状胚胎。第14天子宫腺体发育适中，有丝状胎盘。总而言之，这些结果表明，子宫内膜腺及其分泌物是围植入期胚胎存活和发育所必需的。
Endometrial glands secrete molecules hypothesized to support conceptus growth and development. In sheep, endometrial gland morphogenesis occurs postnatally and can be epigenetically ablated by neonatal progestin exposure. The resulting stable adult uterine gland knockout (UGKO) phenotype was used here to test the hypothesis that endometrial glands are required for successful pregnancy. Mature UGKO ewes were bred repeatedly to fertile rams, but no pregnancies were detected by ultrasound on Day 25. Day 7 blastocysts from normal superovulated ewes were then transferred synchronously into Day 7 control or UGKO ewes. Ultrasonography on Days 25-65 postmating indicated that pregnancy was established in control, but not in UGKO ewes. To examine early uterine-embryo interactions, four control and eight UGKO ewes were bred to fertile rams. On Day 14, their uteri were flushed. The uterus of each control ewe contained two filamentous conceptuses of normal length. Uteri from four UGKO ewes contained no conceptus. Uteri of three UGKO ewes contained a single severely growth-retarded tubular conceptus, whereas the remaining ewe contained a single filamentous conceptus. Histological analyses of these uteri revealed that endometrial gland density was directly related to conceptus survival and developmental state. Day 14 UGKO uteri that were devoid of endometrial glands did not support normal conceptus development and contained either no conceptuses or growth-retarded tubular conceptuses. The Day 14 UGKO uterus with moderate gland development contained a filamentous conceptus. Collectively, these results demonstrate that endometrial glands and, by inference, their secretions are required for periimplantation conceptus survival and development.