Electrochemical RNase A Detection Using an Electrode with Immobilized Ferrocenyl Deoxyribooligonucleotide Containing Cytidine Residue
Electrochemical RNase A Detection Using an Electrode with Immobilized Ferrocenyl Deoxyribooligonucleotide Containing Cytidine Residue
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DOI：
10.1002/elan.201300126
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发表时间：
2013-07
期刊：
影响因子：
3
通讯作者：
Shinobu Sato;S. Takenaka
中科院分区：
文献类型：
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作者：
Shinobu Sato;S. Takenaka
将一种含有胞嘧啶碱基和一个核糖核苷酸胞苷的二茂铁基脱氧核糖寡核苷酸（FcODN（rC））固定在金电极上，并将该电极用于检测核糖核酸酶A。使用循环伏安法评估溶液中的RNase A活性，并且发现传感器电极的电流响应随着酶浓度的增加而降低。观察到的检测限极低，为1.0×10−11 g mL−1 RNase A，电流信号变化为15- 90%。RNA酶活性可以是许多疾病的指标;因此，这种探针在医学诊断中具有很大的应用潜力。
A ferrocenyl deoxyribooligonucleotide (FcODN(rC)) with contiguous cytosine bases and a single ribonucleotide, cytidine, was immobilized on a gold electrode, and this electrode was used to detect RNase A. RNase A activity in a solution was assessed using cyclic voltammetry, and it was found that the current response of the sensor electrode decreased with increasing enzyme concentration. An extremely low detection limit of 1.0×10−11 g mL−1 RNase A was observed, with 15–90 % changes in the current signal. RNase activity can be an indicator of a number of diseases; therefore, this probe has great potential for applications in medical diagnostics.