Nicotine exposure affects mother's and pup's nutritional, biochemical, and hormonal profiles during lactation in rats
Nicotine exposure affects mother's and pup's nutritional, biochemical, and hormonal profiles during lactation in rats
复制标题
DOI：
10.1677/joe-09-0430
复制
发表时间：
2010-05-01
影响因子：
4
通讯作者：
Lisboa, P. C.
中科院分区：
文献类型：
--
作者：
Oliveira, E.;Pinheiro, C. R.;Lisboa, P. C.
我们已经证明，哺乳期母体尼古丁暴露对大鼠后代的身体肥胖和荷尔蒙状况有长期影响。在这里，我们研究了这个实验模型中的营养和激素分布。出生后两天，将渗透压微泵植入哺乳期大鼠，随机分为两组：NIC-持续皮下注射组。尼古丁(6 mg/kg/d)灌胃14天，C-生理盐水灌胃。母猪和幼崽在哺乳15天和21天时被宰杀。评估体重和食物摄入量。采集乳汁、血液、内脏脂肪、屠体和肾上腺。所有有意义的数据均为P<；0.05。在尼古丁暴露结束时(15天)，DAMS表现出更高的产奶量、高催乳素血症和更高的血清高密度脂蛋白胆固醇(HDLC)。来自NIC大坝的牛奶具有较高的乳糖浓度和能量含量。尼古丁戒断后(21天)，DAMS大鼠出现低摄食量和高瘦素血症。15日龄NIC仔鼠具有较高的体脂、高密度脂蛋白胆固醇、血清瘦素、皮质酮和肾上腺儿茶酚胺含量，但酪氨酸羟基酶蛋白水平较低。21日龄NIC仔猪具有较高的体蛋白含量和血清球蛋白水平。因此，母体在哺乳期间接触尼古丁会导致母鼠和子代的营养、生化和激素参数发生重要变化。当比较尼古丁暴露组和戒断组时，这些效应的模式明显不同，这可能对先前观察到的编程效应很重要。内分泌学杂志(2010)205,159-170
We have shown that maternal nicotine exposure during lactation has long-lasting effects on body adiposity and hormonal status of rat offspring. Here, we studied the nutritional and hormonal profiles in this experimental model. Two days after birth, osmotic minipumps were implanted in lactating rats divided into two groups: NIC - continuous s.c. infusions of nicotine (6 mg/kg per day) for 14 days and C - saline. Dams and pups were killed at 15 and 21 days of lactation. Body weight and food intake were evaluated. Milk, blood, visceral fat, carcass, and adrenal gland were collected. All the significant data were P < 0.05. At the end of nicotine exposure (15 days), dams presented higher milk production, hyperprolactinemia, and higher serum high-density lipoprotein cholesterol (HDL-C). Milk from NIC dams had higher lactose concentration and energy content. After nicotine withdrawal (21 days), dams showed lower food intake and hyperleptinemia. The 15-day-old NIC pups presented higher total body fat, higher HDL-C, serum leptin, serum corticosterone, and adrenal catecholamine content, but lower tyrosine hydroxylase protein levels. The 21-day-old NIC pups had higher body protein content and serum globulin. Thus, maternal nicotine exposure during lactation results in important changes in nutritional, biochemical, and hormonal parameters in dams and offspring. The pattern of these effects is clearly distinct when comparing the nicotine-exposed group to the withdrawal group, which could be important for the programming effects observed previously. Journal of Endocrinology (2010) 205, 159-170