Preparation and Mounting of Biting Midges of the Genus Culicoides Latreille (Diptera: Ceratopogonidae) to be Observed with a Scanning Electron Microscope
Preparation and Mounting of Biting Midges of the Genus Culicoides Latreille (Diptera: Ceratopogonidae) to be Observed with a Scanning Electron Microscope
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DOI：
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发表时间：
2000
影响因子：
0.6
通讯作者：
M. Ronderos;G. Spinelli;P. Sarmiento
中科院分区：
文献类型：
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作者：
M. Ronderos;G. Spinelli;P. Sarmiento
技术倾向于保存原始形态和安装的库蠓种的扫描电镜，特别是幼虫和成人，进行了讨论。干燥的成虫干直接安装在银膏允许完美的观察翅膀。成人保存在酒精中，脱水苯酚饱和的无水酒精，在酒精70%，并安装在碳带，允许彻底观察其余的结构。对于幼虫，戊巴比妥是最有效的放松药物。苯酚在无水乙醇中的饱和溶液作为固定剂和澄清剂，是少数几种既适合于光学显微镜观察又适合于扫描电镜观察的固定剂之一。
Techniques tending to the preservation of the original morphology and with the mounting of species of Culicoides for SEM, especially larvae and adults, are discussed. Dried adults dry mounted directly on silver paste allowed the perfect observation of the wings. Adults preserved in alcohol, dehydrated in phenol saturated in absolute alcohol, washed in alcohol 70%, and mounted on carbon tape, permitted a thorough observation of the rest of the structures. With regard to larvae, Nembutal was the most efficient of the relaxing drugs used. Phenol saturated in absolute alcohol acts as fixative and dehydrator, being one of the few fixatives which is apt for the observation not only with light microscope but also with SEM.