Bifunctional Polyoxometalates for Planar Gold Surface Nanostructuration and Protein Immobilization
Bifunctional Polyoxometalates for Planar Gold Surface Nanostructuration and Protein Immobilization
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DOI：
10.1021/jp3031623
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发表时间：
2012-06
影响因子：
3.7
通讯作者：
D. Mercier;S. Boujday;C. Annabi;R. Villanneau;C. Pradier;A. Proust
中科院分区：
文献类型：
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作者：
D. Mercier;S. Boujday;C. Annabi;R. Villanneau;C. Pradier;A. Proust
通过将聚氧乙烯酸盐（POM）（NBu 4）3[PW 11 O39 {（SiC 6 H4 NH 2）2 O}]共价键合到平面金表面上，成功地实现了表面纳米结构化。为此，合成了两个末端氨基官能化的POM，并与吸附在金上的巯基十一烷酸自组装单层反应。这些胺封端的POM大阴离子被证明是非常有效的纳米结构剂。使用偏振调制红外反射吸收光谱（PM-IRRAS），光电子能谱（XPS），和原子力显微镜（AFM），条件进行了优化，阐述了一个致密的和分散良好的层的POM，留下一个“自由”胺功能的进一步连接的蛋白质。因此，抗兔免疫球蛋白（抗rIgG）的POM结构层上接枝，并通过使用石英晶体微天平与耗散测量（QCM-D）测试其特异性目标，兔免疫球蛋白（rIgG）的识别。识别是好的和高度speci.
Surface nanostructuration was successfully achieved by binding the polyoxometalate (POM) (NBu4)3[PW11O39{(SiC6H4NH2)2O}] covalently onto planar gold surfaces. To do so, POMs functionalized with two terminal amino groups were synthesized and reacted with a mercaptoundecanoic acid self-assembled monolayer adsorbed on gold. These amine-terminated POM macroanions proved to be remarkably efficient as nanostructuring agents. Using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), photoelectron spectroscopy (XPS), and atomic force microscopy (AFM), conditions were optimized to elaborate a dense and well-dispersed layer of POMs, leaving a “free” amine function for the further linkage of proteins. Antirabbit immunoglobulins (anti-rIgGs) were thus grafted on the POM-structured layer, and the recognition of their specific target, rabbit immunoglobulin (rIgGs), was tested by using a quartz crystal microbalance with dissipation measurement (QCM-D). The recognition was good and highly speci...