Synthesis, characterization and toxicological evaluation of iron oxide nanoparticles in human lung alveolar epithelial cells
Synthesis, characterization and toxicological evaluation of iron oxide nanoparticles in human lung alveolar epithelial cells
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DOI：
10.1016/j.colsurfb.2014.06.064
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发表时间：
2014-10-01
影响因子：
5.8
通讯作者：
Al-Khedhairy, Abdulaziz A.
中科院分区：
文献类型：
--
作者：
Dwivedi, Sourabh;Siddiqui, Maqsood A.;Al-Khedhairy, Abdulaziz A.
本研究旨在表征合成的氧化铁纳米颗粒（Fe 3 O 4-NPs），并评估其对人肺泡上皮细胞（A-549）的细胞毒性和氧化应激。采用X射线衍射、透射电子显微镜、动态光散射和原子力显微镜对Fe 3 O 4-NPs进行了表征。发现Fe 3 O 4-NPs的形态是可变的，尺寸范围为36 nm。将A-549细胞暴露于Fe 3 O 4-NPs（10-50 μ g/ml浓度）24小时。暴露后，评价了细胞毒性试验（3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四唑，MTT;中性红摄取，NRU;和细胞形态学）和氧化应激（脂质过氧化，LPO和谷胱甘肽，GSH）。此外，细胞内活性氧（ROS）的产生和线粒体膜电位（MMP）也进行了研究。MIT和NRU试验显示A-549细胞的细胞活力呈浓度依赖性降低。Fe 3 O 4-NPs暴露的细胞也改变了细胞的正常形态。此外，细胞显示出显著的氧化应激诱导。这通过LPO和ROS产生的增加以及GSH水平和MMP的降低来证实。我们的研究结果表明，Fe 3 O 4-NPs诱导的细胞毒性可能是通过氧化应激和ROS的产生在A-549细胞介导的。(C)2014爱思唯尔有限公司版权所有。
The present investigation was aimed to characterize the synthesized iron oxide nanoparticles (Fe3O4-NPs) and to assess their cytotoxicity and oxidative stress in human lung alveolar epithelial cells (A-549). Fe3O4-NPs were characterized by X-ray diffraction, transmission electron microscopy, dynamic light scattering, and atomic force microscopy. The morphology of the Fe3O4-NPs was found to be variable with a size range of 36 nm. A-549 cells were exposed to Fe3O4-NPs (10-50 mu g/ml concentrations) for 24 h. Post exposure, cytotoxicity assays (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT; neutral red uptake, NRU; and cellular morphology) and oxidative stress (lipid peroxidation, LPO and glutathione, GSH) were evaluated. Further, intracellular reactive oxygen species (ROS) generation and mitochondrial membrane potential (MMP) were also studied. MIT and NRU assays revealed a concentration-dependent decrease in the cell viability of A-549 cells. Fe3O4-NPs exposed cells also altered the normal morphology of the cells. Furthermore, the cells showed significant induction of oxidative stress. This was confirmed by the increase in LPO and ROS generation, and the decrease in the GSH level and MMP. Our results demonstrated that Fe3O4-NPs induced cytotoxicity is likely to be mediated through the oxidative stress and ROS generation in A-549 cells. (C) 2014 Elsevier B.V. All rights reserved.