Formation of vessel-like channel using alginate fiber as a sacrificial structure
Formation of vessel-like channel using alginate fiber as a sacrificial structure
复制标题
DOI：
10.1109/memsys.2017.7863477
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
K. Nishimura;N. Mori;Y. Morimoto;S. Takeuchi
中科院分区：
文献类型：
--
作者：
K. Nishimura;N. Mori;Y. Morimoto;S. Takeuchi
本研究报道了一种用于在细胞胶原凝胶中灌流培养液的环状血管状通道的制作方法。我们使用海藻酸纤维作为牺牲性结构来建造环状的血管状通道。通过将环状海藻酸纤维与细胞胶原凝胶中的连接端口整合在一起，我们形成了可管连接的环状通道。最后，我们将内皮细胞种植到通道的内壁上，以构建包覆内皮细胞的血管样通道。因此，我们通过环形的管状通道实现了对培养基的灌流。我们相信，这种方法将有助于体外构建具有复杂形状的血管状通道的三维(3D)组织。
This research reports a method to fabricate a loop-shaped vessel-like channel for perfusion of culture medium in a cell-laden collagen gel. We used alginate fibers as sacrificial structures for construction of loop-shaped vessel-like channels. By integration of a loop-shaped alginate fiber with connection ports in a cell-laden collagen gel, we formed a tube-connectable loop-shaped channel. Finally, we seeded endothelial cells onto inner wall of the channel to fabricate vessel-like channel coated with endothelial cells. As a result, we achieved perfusion of culture medium via the loop-shaped vessel-like channel. We believe that this method will be useful for in vitro construction of three-dimensional (3D) tissues with complex-shaped vessel-like channels.