AtMYB44 positively modulates disease resistance to Pseudomonas syringae through the salicylic acid signalling pathway in Arabidopsis.
AtMYB44 positively modulates disease resistance to Pseudomonas syringae through the salicylic acid signalling pathway in Arabidopsis.
复制标题
DOI：
10.1071/fp12253
复制
发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Baohong Zou;Zhen-hua Jia;S-M Tian;Xiaomen Wang;Zhenhua Gou;B. L.;Hansong Dong
中科院分区：
文献类型：
--
作者：
Baohong Zou;Zhen-hua Jia;S-M Tian;Xiaomen Wang;Zhenhua Gou;B. L.;Hansong Dong
植物MYB转录因子与植物对生物和非生物胁迫的抗性有关。在这里，我们表明，R2-R3 MYB转录因子，AtMYB 44，在植物防御反应的细菌病原体假单胞菌pv.番茄DC 3000（PstDC 3000）为材料，AtMYB 44的表达上调病原体感染和防御相关的植物激素治疗。过表达AtMYB 44（35 S-Ms）的转基因植株在感染PstDC 3000的叶片中表现出更高水平的PR 1基因表达、细胞死亡、胼胝质沉积和过氧化氢（H2 O2）积累。因此，35 S-M株系对PstDC 3000表现出增强的抗性。与此相反，atmyb 44 T-DNA插入突变体更容易受到PstDC 3000的感染，并表现出减少PR 1基因的表达。利用35 S-M株系与NahG转基因植株和病程相关基因非表达突变体（npr 1 -1）杂交构建的双突变体，证明了35 S-M植株中PR 1基因表达增强和PstDC 3000抗性主要通过水杨酸信号传导途径发生。
Plant MYB transcription factors are implicated in resistance to biotic and abiotic stresses. Here, we demonstrate that an R2-R3 MYB transcription factor, AtMYB44, plays a role in the plant defence response to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (PstDC3000). The expression of AtMYB44 was upregulated upon pathogen infection and treatments with defence-related phytohormones. Transgenic plants overexpressing AtMYB44 (35S-Ms) exhibited greater levels of PR1 gene expression, cell death, callose deposition and hydrogen peroxide (H2O2) accumulation in leaves infected with PstDC3000. Consequently, 35S-M lines displayed enhanced resistance to PstDC3000. In contrast, the atmyb44 T-DNA insertion mutant was more susceptible to PstDC3000 and exhibited decreased PR1 gene expression upon infection. Using double mutants constructed via crosses of 35S-M lines with NahG transgenic plants and nonexpressor of pathogenesis-related genes1 mutant (npr1-1), we demonstrated that the enhanced PR1 gene expression and PstDC3000 resistance in 35S-M plants occur mainly through the salicylic acid signalling pathway.