IMPAIRED RECRUITMENT AND DIFFERENTIATION OF OSTEOCLAST PROGENITORS BY OSTEOCALCIN-DEPLETE BONE IMPLANTS
IMPAIRED RECRUITMENT AND DIFFERENTIATION OF OSTEOCLAST PROGENITORS BY OSTEOCALCIN-DEPLETE BONE IMPLANTS
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DOI：
10.1016/0045-6039(87)90479-9
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发表时间：
1987-09-01
期刊：
影响因子：
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通讯作者：
LIAN, JB
中科院分区：
文献类型：
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作者：
GLOWACKI, J;LIAN, JB
这是一个实验系统的报告，以研究骨吸收破骨细胞的分化，并证明骨钙素，细胞外骨特异性成分，是必要的招募破骨细胞祖细胞。失活骨颗粒（BP）的皮下植入促进破骨细胞的募集和分化，破骨细胞吸收BP。在以前的研究中，我们通过组织形态计量学分析表明，骨钙素缺乏的BP仅被吸收60%，与正常BP一样。在这项研究中，这种差异的机制进行了研究，通过测量正常和骨钙素缺乏BP的骨吸收细胞的招募，分化和活性。移植后不久，单核细胞被吸引到对照BP。与此形成鲜明对比的是，在第5天，骨钙素缺陷BP周围的细胞结构被抑制，植入物内的单核细胞非常少（对照细胞结构的35%）。在植入正常的BP，抗酒石酸酸性磷酸酶阳性多核细胞是明显的第5天，很少出现在植入骨钙素耗尽BP，甚至到第12天。抗酒石酸酸性磷酸酶活性的骨钙素缺乏的骨颗粒标本的匀浆中的量不仅落后于对照组，但从未达到最大活性的控制BP标本。这些数据支持骨钙素可能在骨吸收细胞的募集和/或激活中起基质信号作用的假设。
This is a report of an experimental system to study differentiation of bone-resorbing osteoclasts and demonstrates that osteocalcin, an extracellular bone-specific component, is necessary for the recruitment of osteoclast progenitor cells. The subcutaneous implantation of devitalized bone particles (BPs) elicits the recruitment and differentiation of osteoclasts that resorb the BPs. In a previous study, we showed by histomorphometric analysis that BPs that were deficient in osteocalcin were resorbed only 60% as well as normal BPs. In this study, the mechanism of this difference was investigated by measurements of recruitment, differentiation and activity of bone resorbing cells by normal and osteocalcin-deficient BP. Mononuclear cells were attracted to control BPs soon after implantation. In dramatic contrast, cellularity was depressed around osteocalcin-deficient BPs with very few mononuclear cells within the implant on day 5 (35% of control cellularity). In implants of normal BPs, tartrate-resistant acid phosphatase-positive multinucleated cells were evident by day 5; very few appeared in implants of osteocalcin-deplete BPs even by day 12. The amount of tartrate-resistant acid phosphatase activity in homogenates of the osteocalcin-deficient bone particle specimens not only lagged behind controls but never reached the maximum activity of control BP specimens. These data support the hypothesis that osteocalcin may function as a matrix signal in the recruitment and/or activation of cells for bone resorption.