Cytokine production by naive and primary effector CD4+ T cells exposed to norepinephrine
Cytokine production by naive and primary effector CD4+ T cells exposed to norepinephrine
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DOI：
10.1006/brbi.2000.0603
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发表时间：
2000-12-01
影响因子：
15.1
通讯作者：
Sanders, VM
中科院分区：
文献类型：
--
作者：
Ramer-Quinn, DS;Swanson, MA;Sanders, VM
我们最近发现，Th 1细胞克隆，而不是Th 2细胞，表达功能性β 2肾上腺素能受体（β 2 AR），无论是去甲肾上腺素或β 2 AR激动剂特布他林刺激该受体调节Th 1细胞因子的水平产生。在本研究中，我们发现去甲肾上腺素和特布他林刺激β 2 AR，以降低来自Balb/C或DO 11.10转基因小鼠的新鲜分离的小鼠脾脏初始CD 4（+）T细胞产生的IL-2水平，并用抗CDS和抗CD 28 mAb多克隆激活。相比之下，当在再刺激时加入去甲肾上腺素、特布他林或cAMP类似物时，初级效应Th 1和Th 2细胞产生的细胞因子水平不受影响。这些结果表明，CD 4（+）T细胞亚群之间存在的差异，肾上腺素能受体表达水平，cAMP的反应性，细胞分化的阶段，或上述的组合。(C)北京大学出版社.
We recently showed that clones of Th1 cells, but not Th2 cells, expressed a functional beta-2-adrenergic receptor (beta (2)AR) and that either norepinephrine or the beta (2)AR agonist terbutaline stimulated this receptor to modulate the level of Th1 cytokines produced. In the present study, we show that norepinephrine and terbutaline stimulate the beta (2)AR to decrease the level of IL-2 produced by freshly isolated murine splenic naive CD4(+) T cells from either Balb/C or DO 11.10 transgenic mice and activated polyclonally with anti-CDS and anti-CD28 mAbs. In contrast, the level of cytokines produced by primary effector Th1 and Th2 cells were unaffected when norepinephrine, terbutaline, or cAMP analogs were added at the time of restimulation. These results suggest that a diversity exists among CD4(+) T-cell subsets with respect to the level of adrenergic receptor expression, responsiveness to cAMP, stage of cell differentiation, or a combination of the above. (C) 2000 Academic Press.