The isolation of two juvenile hormone-inducible genes in Drosophila melanogaster
The isolation of two juvenile hormone-inducible genes in Drosophila melanogaster
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DOI：
10.1006/dbio.2000.9800
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发表时间：
2000-08-15
影响因子：
2.7
通讯作者：
Berger, EM
中科院分区：
文献类型：
--
作者：
Dubrovsky, EB;Dubrovskaya, VA;Berger, EM
保幼激素是昆虫发育和生殖成熟的重要调节因子。尽管JH作用的分子机制尚不清楚，但越来越多的间接证据表明JH直接调节基因表达。然而，在缺乏JH靶基因的情况下，这一建议仍然是推测性的。培养的果蝇S2细胞已用于鉴定表达受JH调节的基因。采用差异显示，我们确定了几个基因的转录积累在细胞中处理的JH激动剂烯虫腈。其中两个基因-JhI-1和JhI-26-被克隆并详细表征。对于这两个基因，成绩单表现出快速和特异性的诱导，无论是甲氧普烯或JHIII的存在下，但不是在其他类似的化学结构的生物活性化合物的存在下。JhI-1和JhI-26 RNA的积累需要持续的激素存在。两个JH诱导基因的发育表达对应于体内JH的丰度谱。此外，局部甲氧普烯应用于脓疱导致JhI-1和JhI-26转录物的异位积累。(C)北京大学出版社.
Juvenile hormone (JH) is an important regulator of both insect development and reproductive maturation. Although the molecular mechanism of JH action is not yet known, there is growing circumstantial evidence that JH directly regulates gene expression. In the absence of a JH target gene, however, this suggestion has remained speculative. Cultured Drosophila S2 cells have been used to identify genes whose expression is regulated by JH. Employing differential display we identified several genes whose transcripts accumulate in cells treated with the JH agonist methoprene. Two of the genes-JhI-1 and JhI-26-were cloned and characterized in detail. For both genes, transcripts showed rapid and specific induction in the presence of either methoprene or JHIII, but not in the presence of other biologically inactive compounds of similar chemical structure. Accumulation of JhI-1 and JhI-26 RNAs requires continuous hormone presence. The developmental expression of the two JH-inducible genes corresponds to the abundance profile of JH in vivo. Furthermore, topical methoprene application to pupae leads to the ectopic accumulation of JhI-1 and JhI-26 transcripts. (C) 2000 Academic Press.