A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana.
A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana.
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DOI：
10.1093/gigascience/gix035
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发表时间：
2017-07-01
期刊：
影响因子：
9.2
通讯作者：
Blaxter M
中科院分区：
文献类型：
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作者：
Nowell RW;Elsworth B;Oostra V;Zwaan BJ;Wheat CW;Saastamoinen M;Saccheri IJ;Van't Hof AE;Wasik BR;Connahs H;Aslam ML;Kumar S;Challis RJ;Monteiro A;Brakefield PM;Blaxter M
树蛙蝶Bicyclus anynana（英语：Bicyclus anynana）是鳞翅目生态学、发育和进化研究的模式生物。在这里，我们提出了一个草案的基因组序列B。anynana作为一个基因组学资源，为当前和未来的研究，这一重要的模式物种。使用来自近交系雌性的DNA构建具有350 bp至20 kb范围内的插入片段大小的七个文库，并使用Illumina和PacBio技术两者进行测序;将128 Gb的原始Illumina数据过滤至124 Gb并组装至475 Mb的最终大小（100 ×260组装覆盖率）。使用配对、转录组和PacBio数据将重叠群支架化成10800个序列，其中N50为638 kb（最长支架5 Mb）。基因组由26%的重复元件组成，共编码22642个预测的蛋白质编码基因。恢复的核心后生动物基因的BUSCO集几乎是完全的（98%）。总的来说，这些指标与其他最近发表的鳞翅目基因组比较。我们报告了一个高质量的草案基因组序列的Bicyclus anynana。基因组组装和注释的基因模型可在LepBase（http：//ensembl.lepbase.org/index.html）获得。
The mycalesine butterfly Bicyclus anynana, the “Squinting bush brown,” is a model organism in the study of lepidopteran ecology, development, and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species. Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology; 128 Gb of raw Illumina data was filtered to 124 Gb and assembled to a final size of 475 Mb (∼×260 assembly coverage). Contigs were scaffolded using mate-pair, transcriptome, and PacBio data into 10 800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements and encodes a total of 22 642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes. We report a high-quality draft genome sequence for Bicyclus anynana. The genome assembly and annotated gene models are available at LepBase (http://ensembl.lepbase.org/index.html).