PSAP gene variants and haplotypes reveal significant effects on carcass and meat quality traits in Chinese Simmental-cross cattle
PSAP gene variants and haplotypes reveal significant effects on carcass and meat quality traits in Chinese Simmental-cross cattle
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PSAP基因变异和单倍型揭示对中国西门塔尔杂交牛胴体和肉质性状的显着影响
DOI：
10.5194/aab-59-461-2016
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发表时间：
2016-11-14
影响因子：
1.8
通讯作者：
Yang, Runjun
中科院分区：
文献类型：
--
作者：
Guo, Pengcheng;Zhao, Zhihui;Yang, Runjun
摘要。Prosaposin （PSAP）是四种小溶酶体糖蛋白的前体蛋白，在肉牛肌肉生长发育中起重要作用。本研究旨在探讨PSAP基因多态性与中国西门特杂交牛胴体组成和肉质性状的关系。通过直接DNA测序，在380头肉牛PSAP基因的内含子10和外显子11中鉴定出6个snp，并测定了47个胴体组成和肉质性状。利用统计学方法分析了PSAP基因变异与经济性状的关系。结果显示,一块- 65 G > ,一块- 313 c > T, e11 - 87 c > T和e11 - 93 c > G与胴体性状显著相关,如穿的百分比,脂肪覆盖率,尸体深度,后腿宽度,一块- 162 c > T和一块- 274 c > T和大理石花纹评分特征显著相关。此外，GCCCGC单倍型个体的屠宰重、屠宰率和肋眼面积显著高于其他个体（p < 0.05）
Abstract. Prosaposin (PSAP) is the precursor protein of four small lysosomal glycoproteins and plays vital roles in muscle growth and development of beef cattle. The aim of this study is to evaluate the association between PSAP gene polymorphisms with carcass composition and meat quality traits of Chinese Simmental-cross cattle. In the present study, six SNPs were identified within intron 10 and exon 11 of the PSAP gene from 380 beef cattle by direct DNA sequencing, and 47 traits of carcass composition and meat quality were measured in the studied population. Then, the relationship between variants of PSAP gene with economic traits was analyzed using statistical methods. The result revealed that I10-65G > A, I10-313C > T, E11-87C > T, and E11-93C > G were significantly associated with carcass traits, such as dressing percentage, fat coverage rate, carcass depth, and hind leg width, and I10-162C > T and I10-274C > T were significantly associated with marbling score traits. In addition, the individuals with GCCCGC haplotype showed significant higher dressed weight, dressing percentage and rib eye area (p