Detecting viral genomes in the female urinary microbiome
Detecting viral genomes in the female urinary microbiome
复制标题
DOI：
10.1099/jgv.0.001097
复制
发表时间：
2018-08-01
影响因子：
3.8
通讯作者：
Putonti, Catherine
中科院分区：
文献类型：
--
作者：
Garretto, Andrea;Thomas-White, Krystal;Putonti, Catherine
病毒是人类微生物区系中含量最丰富的成分。最近的证据发现，女性膀胱内存在丰富多样的病毒，包括噬菌体和真核病毒。我们对30名女性的膀胱微生物组进行了全基因组测序：10名无症状的健康女性和20名膀胱过度活跃的女性。这些元基因组包括代表人类、细菌和病毒DNA的序列。然而，这项分析特别关注病毒序列。利用生物信息学工具virmine，我们发现了噬菌体和真核病毒JC多瘤病毒的序列片段和完整的基因组。这里使用的方法是概念的关键证明：低生物量膀胱微生物群内的病毒种群的基因组可以通过整个微生物群落的全基因组测序来重建。
Viruses are the most abundant component of the human microbiota. Recent evidence has uncovered a rich diversity of viruses within the female bladder, including both bacteriophages and eukaryotic viruses. We conducted whole-genome sequencing of the bladder microbiome of 30 women: 10 asymptomatic 'healthy' women and 20 women with an overactive bladder. These metagenomes include sequences representative of human, bacterial and viral DNA. This analysis, however, focused specifically on viral sequences. Using the bioinformatic tool virMine, we discovered sequence fragments, as well as complete genomes, of bacteriophages and the eukaryotic virus JC polyomavirus. The method employed here is a critical proof of concept: the genomes of viral populations within the low-biomass bladder microbiota can be reconstructed through whole-genome sequencing of the entire microbial community.