Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis
Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis
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DOI：
10.1007/bf02977333
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发表时间：
2007-12-01
影响因子：
6.7
通讯作者：
Choi, Hyung-Kyoon
中科院分区：
文献类型：
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作者：
Shin, Yoo-Soo;Bang, Kyong-Hwan;Choi, Hyung-Kyoon
采用h -1核磁共振光谱和多变量分析技术对新鲜人参根龄进行指纹图谱分析。人参鲜根水提物的h -1 nmr谱在脂肪族（0-3 ppm）、糖区（3-6 ppm）和芳香区（6-9 ppm）存在不同的峰。使用主成分分析（PCA）进行代谢组学分析，可以将各种代谢物获得的大量H-1-NMR数据集简化为PC1、PC2和PC3。二维分值图显示，这三个成分在不同根龄下存在明显的分离，解释了总方差的89.6%。典型判别分析从核磁共振结果中鉴定出不同年龄的人参根，判别准确率超过89.9%。上述结果表明，h -1核磁共振与主成分分析相结合为不同年龄新鲜人参根的鉴别和质量控制提供了一种很有前景的工具。
Fingerprinting analysis of fresh ginseng according to root age was performed using H-1-NMR spectroscopy and multivariate analysis techniques. Various peaks were detected in the aliphatic (0-3 ppm), sugar (3-6 ppm), and aromatic (6-9 ppm) regions of the H-1-NMR spectra of the water extracts of fresh ginseng root. The use of principal components (PCs) analysis (PCA) for metabolomic profiling allowed the large H-1-NMR data set obtained for various metabolites to be reduced to PC1, PC2, and PC3. Two dimensional score plots showed clear separations with these three components at different roots ages, and explained 89.6% of the total variance. Canonical discriminant analysis identified the ginseng roots at various ages from the NMR results with over 89.9% discrimination accuracy. These results indicate that the combination of H-1-NMR and PCA provides a very promising tool for the authentication and quality control of fresh ginseng roots at different ages.