Molecular characterization of integrons in non-typhoid Salmonella serovars isolated in Japan:: description of an unusual class 2 integron
Molecular characterization of integrons in non-typhoid Salmonella serovars isolated in Japan:: description of an unusual class 2 integron
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DOI：
10.1093/jac/dkh534
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发表时间：
2005-03-01
影响因子：
5.2
通讯作者：
Shimamoto, T
中科院分区：
文献类型：
--
作者：
Ahmed, AM;Nakano, H;Shimamoto, T
目的：在日本非伤寒沙门氏菌血清型中鉴定1类和2类整合子，并监测多重耐药沙门氏菌的传播。方法：对105株沙门氏菌进行检测。采用微量肉汤稀释法测定了一系列抗生素对这些分离株的MIC值。结果：PCR和DNA测序分析显示，除一种新的2类整合子外，还存在7种1类整合子。1类整合子中鉴定的基因盒如下：aadA 1、aadA 2和aadA 5，其赋予对链霉素和壮观霉素的抗性; aadB，其赋予对庆大霉素、卡那霉素和妥布霉素的抗性; dfrA 1和dfrA 17，其赋予对甲氧苄啶的抗性; bla（PSE-1），其赋予对氨苄青霉素的抗性; catB 3，其赋予对氯霉素的抗性;和SAT 1，其赋予对链丝菌素的抗性。两株多重耐药的S.本研究对鼠伤寒沙门氏菌DT 104进行了表征。对2类整合子进行DNA测序，发现1个整合子中含有sat、sat 1和aadA 1基因盒。结论：本研究对非伤寒沙门菌血清型1类整合子中的耐药基因盒进行了鉴定，并发现了一种新的2类整合子。
Objectives: To characterize class 1 and class 2 integrons among non-typhoid Salmonella enterica serovars in Japan, and also to monitor the spread of the multidrug-resistant S. enterica serovar Typhimurium DT104.Methods: A total of 105 Salmonella isolates were included in this study. The broth microdilution method was used to determine the MIC values of a range of antibiotics for these isolates. PCR and DNA sequencing were used for screening and characterization of class 1 and class 2 integrons.Results: PCR sequencing analysis revealed the presence of seven profiles of class 1 integrons in addition to a new type of class 2 integron. The identified gene cassettes within class 1 integrons were as follows; aadA1, aadA2 and aadA5, which confer resistance to streptomycin and spectinomycin; aadB, which confers resistance to gentamicin, kanamycin and tobramycin; dfrA1 and dfrA17, which confer resistance to trimethoprim; bla(PSE-1), which confers resistance to ampicillin; catB3, which confers resistance to chloramphenicol; and sat1, which confers resistance to streptothricin. Two strains of the multidrug-resistant S. Typhimurium DT104 were characterized in this study. DNA sequencing of class 2 integrons identified one with an unusual array of gene cassettes, sat, sat1 and aadA1.Conclusions: In this study, we characterized the antibiotic resistance gene cassettes within class 1 integrons in different isolates of non-typhoid Salmonella serovars, and we also identified a new type of class 2 integron.