Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma
Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma
复制标题
使用丝素蛋白-壳聚糖支架构建组织工程角膜基质
DOI：
10.1159/000355944
复制
发表时间：
2013-01-01
影响因子：
2.7
通讯作者：
Liu, Ping
中科院分区：
文献类型：
--
作者：
Guan, Linan;Ge, Hongyan;Liu, Ping
目的：以丝素蛋白（SF）和壳聚糖（CS）为支架材料，构建一种可替代角膜基质的支架材料，同时保持支架材料的生物学特性。方法：以SF和CS为材料合成组织工程生物支架，构建器官型角膜基质。体外培养原代兔角膜上皮细胞和角膜基质细胞。使用角质形成细胞来评估SF-CS（SFCS）共混物的细胞毒性，其通过细胞计数试剂盒-8测定来确定。角膜板层支架的开发与序贯培养技术，形成细胞支架结构。在15只新西兰白色家兔中进行了植入试验。通过光学显微镜和电子显微镜分析角膜替代物。结果如下：重建的板层角膜与天然组织相当，角膜上皮细胞中K3/12表达水平较高，基质细胞中波形蛋白表达水平较高;此外，可以通过组织学方法区分细胞的形态和位置。植入后12周，移植椎间盘内或周围无任何免疫反应迹象。结论：SFCS支架材料是一种较理想的角膜组织工程材料。(C)2013 S. Karger AG，巴塞尔
Purpose: To construct a scaffold using silk fibroin (SF) and chitosan (CS) that could replace the corneal stroma with the biological characteristics of the scaffold materials still intact. Methods: To develop an organotypic corneal stroma, SF and CS were chosen to synthesise the tissue-engineered bioscaffold. We cultured primary rabbit corneal epithelial cells and corneal stromal cells in vitro. Keratocytes were used to assess cytotoxicity on SF-CS (SFCS) blends, which was determined by a Cell Counting Kit-8 assay. The corneal lamellar scaffolds were developed with sequential culture techniques to form cell-scaffold constructs. Implantation was tested in 15 New Zealand White rabbits. The corneal substitutes were analysed by light and electron microscopy. Results: The reconstructed lamellar cornea was comparable to native tissue, with high levels of K3/12 expression in the corneal epithelial cells and vimentin in the stromal cells; moreover, the morphology and the position of the cells could be distinguished by histological methods. There was no sign of any immune reaction in or around the transplanted discs 12 weeks after implantation. Conclusion: A SFCS scaffold might be a suitable blend for corneal tissue engineering. (C) 2013 S. Karger AG, Basel