Synthesis and characterization of NIR-responsive Aurod@pNIPAAm-PEGMA nanogels as vehicles for delivery of photodynamic therapy agents.
Synthesis and characterization of NIR-responsive Aurod@pNIPAAm-PEGMA nanogels as vehicles for delivery of photodynamic therapy agents.
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近红外响应 Aurod@pNIPAAm-PEGMA 纳米凝胶作为光动力治疗药物递送载体的合成和表征
DOI：
10.1186/1556-276x-8-4
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发表时间：
2013-01-02
影响因子：
--
通讯作者：
Yang AQ
中科院分区：
文献类型：
--
作者：
Shang T;Wang CD;Ren L;Tian XH;Li DH;Ke XB;Chen M;Yang AQ
通过在金纳米棒（AuNRs）表面生长PEGMA单分子层，然后原位聚合交联N-异丙基丙烯酰胺（NIPAAm）和聚乙二醇甲基丙烯酸酯（PEGMA），制备了具有近红外（NIR）响应的Aurod@pNIPAAm-PEGMA纳米凝胶。采用紫外-可见光谱、拉曼光谱、傅立叶变换红外光谱和透射电子显微镜对AuNR和Aurod@pNIPAAm-PEGMA纳米凝胶进行了表征。Aurod@pNIPAAm-PEGMA纳米凝胶的低临界溶解温度可以通过改变NIPAAm/PEGMA的摩尔比来调节。以酞菁锌（ZnPc 4）为药物模型，研究了Aurod@pNIPAAm-PEGMA纳米凝胶的近红外介导的药物释放行为。结果表明，负载ZnPc 4后，能保持产生单线态氧的能力，并对Hela细胞有明显的光动力学治疗作用。这表明Aurod@pNIPAAm-PEGMA纳米凝胶具有作为PDT药物载体的潜力，可能对肿瘤治疗做出贡献。
A near-infrared (NIR)-responsive Aurod@pNIPAAm-PEGMA nanogel was synthesized in two steps, growing a PEGMA monolayer on the surface of gold nanorods (AuNRs), followed byin situpolymerization and cross-linking ofN-iso-propylacrylamide (NIPAAm) and poly-(ethylene glycol)-methacrylate (PEGMA). The AuNRs and Aurod@pNIPAAm-PEGMA nanogel were characterized by UV–vis spectroscopy, Raman spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy, respectively. The lower critical solution temperature of the Aurod@pNIPAAm-PEGMA nanogel could be tuned by changing the molar ratio of NIPAAm/PEGMA. The NIR-mediated drug release behavior of the Aurod@pNIPAAm-PEGMA nanogel was studied with zinc phthalocyanines (ZnPc4) as a drug model. It was also demonstrated that the loaded ZnPc4could keep the capability of generating singlet oxygen, and thein vitrostudy showed a great photodynamic therapy (PDT) effect on Hela cells. It thus indicated the potential of this Aurod@pNIPAAm-PEGMA nanogel for application as a drug carrier in PDT, which might make contributions to oncotherapy.