Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe
Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe
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DOI：
10.1007/s00294-005-0024-3
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发表时间：
2005-11-01
期刊：
影响因子：
2.5
通讯作者：
Kohli, J
中科院分区：
文献类型：
--
作者：
Doll, E;Molnar, M;Kohli, J
In S.检测到了rec15异常孢子形态、孢子活力降低和减数分裂重组严重减少的粟酒裂殖酵母菌株突变体。遗传和细胞学分析确定频繁中断减数分裂后的第一次分裂，和不分离I，在突变体中的主要分离错误。rec15减数分裂Ⅰ染色体分离不是随机的，这表明存在一个备份系统来正确分离非交叉染色体。减数分裂进程的时间过程实验的分析表明，主要的减数分裂事件，如减数分裂前的DNA合成，马尾核运动，和第一次减数分裂分裂发生早于rec15比野生型。减数分裂事件的早期发生是早期重组突变体的一个新的观察，并暗示rec15蛋白已经在DNA合成或之前的功能。
In S. pombe strains mutant for rec15 aberrant ascus morphology, reduced spore viability and severe reduction of meiotic recombination was detected. Genetic and cytological analysis identified frequent interruption of meiosis after the first division, and nondisjunction I, as the main segregation errors in the mutant. Chromosome segregation at meiosis I was not random in rec15, suggesting the presence of a backup system for correct segregation of achiasmate chromosomes. The analysis of meiotic progression in time-course experiments revealed that the major meiotic events, such as the onset of premeiotic DNA synthesis, of horse-tail nuclear movement, and of the first meiotic division occurred earlier in rec15 than in wild-type. The early onset of meiotic events is a novel observation for an early recombination mutant and implies a function of rec15 protein already at or before DNA synthesis.