Evidence for ecto-protein kinase activity on the surface of human neutrophils.
Evidence for ecto-protein kinase activity on the surface of human neutrophils.
复制标题
人类中性粒细胞表面胞外蛋白激酶活性的证据。
DOI：
10.1016/s0006-291x(88)81182-3
复制
发表时间：
1988
影响因子：
3.1
通讯作者：
Skubitz,KM
中科院分区：
文献类型：
--
作者：
Dusenbery,KE;Mendiola,JR;Skubitz,KM
最近的研究表明细胞外 ATP 在中性粒细胞功能中可能发挥作用。该报告提供了人类中性粒细胞表面存在外蛋白激酶活性的证据，该活性能够磷酸化内在细胞膜蛋白以及外源蛋白。向中性粒细胞添加细胞外[γ-32P]ATP导致32P快速掺入对胰蛋白酶敏感的细胞蛋白中。贴壁细胞磷酸化外源底物酪蛋白的能力，同时没有蛋白激酶活性释放到上清液中，这为细胞表面相关的蛋白激酶活性提供了进一步的证据。
Recent studies have suggested a possible role for extracellular ATP in neutrophil function. This report provides evidence for the existence of an ecto-protein kinase activity on the surface of human neutrophils capable of phosphorylating intrinsic cell membrane proteins as well as exogenous proteins. Addition of extracellular [γ-32P]ATP to neutrophils resulted in rapid incorporation of32P into cellular proteins that were sensitive to trypsin. The ability of adherent cells to phosphorylate the exogenous substrate casein, while no protein kinase activity was released into the supernate, provided further evidence for a cell surface associated protein kinase activity.