Construction of LRET-Based Nanoprobe Using Upconversion Nanoparticles with Confined Emitters and Bared Surface as Luminophore
Construction of LRET-Based Nanoprobe Using Upconversion Nanoparticles with Confined Emitters and Bared Surface as Luminophore
复制标题
使用具有受限发射器和裸露表面作为发光体的上转换纳米粒子构建基于 LRET 的纳米探针。
DOI：
10.1021/jacs.5b01504
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发表时间：
2015-03-11
影响因子：
15
通讯作者：
Liu, Zhihong
中科院分区：
文献类型：
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作者：
Li, Zhen;Lv, Songwei;Liu, Zhihong
上转换纳米粒子（UCNPs）是发光共振能量转移（LRET）的理想能量供体，已被广泛用于构建纳米探针。为了提高LRET效率，这是目前基于UCNPs的生物测定的限制因素，我们在本文中提出了一种策略，使用具有受限发射体和裸露表面的UCNPs作为发光体，以Ca（2+）作为概念验证目标，构建基于LRET的纳米探针。该结构的上转换纳米粒子（SWUCNPs）具有核-内壳-外壳的结构，其中发射离子（Ln（3+））精确地位于粒子表面附近的内壳层中，该内壳层与外部能量受体足够接近。靶受体（Fluo-4）直接标记在SWUCNP的裸露表面上，这进一步减小了供体-受体距离。我们的策略有助于显着提高LRET的效率，因此提供了一个钙离子检测的灵敏度。所构建的纳米探针结构稳定，具有良好的生物相容性，这确保了在活细胞中的摄取和可靠的观察。纳米探针可用于监测活细胞中胞质溶胶[Ca（2+）]的不同水平。此外，它还可用于小鼠肝组织的Ca（2+）显像。
Upconversion nanoparticles (UCNPs) are promising energy donors for luminescence resonance energy transfer (LRET) and have widely been used to construct nanoprobes. To improve the LRET efficiency, which is currently a limiting factor for UCNPs-based bioassay, we herein propose a strategy to construct LRET-based nanoprobe using UCNPs with confined emitters and bared surface as the luminophore, with Ca(2+) as the proof-of-concept target. The sandwich-structure upconversion nanoparticles (SWUCNPs) are designed with a core-inner shell-outer shell architecture, in which the emitting ions (Ln(3+)) are precisely located in the inner shell near the particle surface, which is close enough to external energy acceptors. The target receptor (Fluo-4) is directly tagged on bared surface of SWUCNPs, which further reduces the donor-to-acceptor distance. Our strategy contributes to significantly improved LRET efficiency and hence affords an ultrahigh sensitivity for Ca(2+) detection. The as-constructed nanoprobe is structurally stable and exhibits good biocompatibility, which ensures uptake and reliable observation in living cells. The nanoprobe can be used for monitoring the different levels of cytosol [Ca(2+)] in living cells. Furthermore, it is applicable in Ca(2+) imaging in mice liver tissues.