Plasma Osteopontin Levels Is Associated with Biochemical Markers of Kidney Injury in Patients with Leptospirosis
Plasma Osteopontin Levels Is Associated with Biochemical Markers of Kidney Injury in Patients with Leptospirosis
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DOI：
10.3390/diagnostics10070439
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发表时间：
2020-07-01
期刊：
影响因子：
3.6
通讯作者：
Hattori, Toshio
中科院分区：
文献类型：
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作者：
Chagan-Yasutan, Haorile;Hanan, Firmanto;Hattori, Toshio
钩端螺旋体病变得严重，死亡率> 10%，并表现为严重的肺损伤伴急性肾损伤。为了解骨桥蛋白（OPN）和半乳糖凝集素-9（Gal-9）在钩端螺旋体病（钩体病）中的病理和诊断作用，对112例钩体病患者的尿液和血液标本进行了骨桥蛋白（OPN）和半乳糖凝集素-9（Gal-9）等肾脏相关生物标志物的检测。全长（FL）-OPN（pFL-OPN）的血浆水平（p < 0.0001），pFL-Gal-9（p < 0.0001）和凝血酶裂解的OPN（p < 0.01），在钩端螺旋体病患者中显著高于健康对照组（n = 30），肾毒性的几个指标水平也是如此：血清胱抑素C（p < 0.0001）、尿N-乙酰-β-氨基葡萄糖苷酶（NAG）/肌酐（p < 0.05）和尿簇蛋白/肌酐（p < 0.05）。pFL-Gal-9水平与pFL-OPN水平呈负相关（r =-0.24，p < 0.05）。pFL-OPN水平与血清胱抑素C（r = 0.41，p < 0.0001）、尿NAG/肌酐（r = 0.35，p < 0.001）、尿簇蛋白/肌酐（r = 0.33，p < 0.01）和尿胱抑素C/肌酐（r = 0.33，p < 0.05）水平正相关。在一组肌酐水平异常高的患者中，观察到血清胱抑素C（p < 0.0001）和pFL-OPN（p < 0.001）水平显著较高。我们的研究结果表明，pFL-OPN反映了钩端螺旋体病患者的肾损伤。
Leptospirosis becomes severe, with a fatality rate of >10%, and manifests as severe lung injury accompanied by acute kidney injury. Using urine and blood samples of 112 patients with leptospirosis, osteopontin (OPN), galectin-9 (Gal-9) and other kidney-related biomarkers were measured to understand the pathological and diagnostic roles of OPN and Gal-9 in leptospirosis. Plasma levels of full-length (FL)-OPN (pFL-OPN) (p < 0.0001), pFL-Gal-9(p < 0.0001) and thrombin-cleaved OPN (p < 0.01) were significantly higher in patients with leptospirosis than in healthy controls (n = 30), as were levels of several indicators of renal toxicity: serum cystatin C (p < 0.0001), urine N-acetyl-P-glucosaminidase (NAG)/creatinine (p < 0.05), and urine clusterin/creatinine (p < 0.05). pFL-Gal-9 levels were negatively correlated with pFL-OPN levels (r = -0.24, p < 0.05). pFL-OPN levels were positively correlated with serum cystatin C (r = 0.41, p < 0.0001), urine NAG/creatinine (r = 0.35, p < 0.001), urine clusterin/creatinine (r = 0.33, p < 0.01), and urine cystatin C/creatinine (r = 0.33, p < 0.05) levels. In a group of patients with abnormally high creatinine levels, significantly higher levels of serum cystatin C (p < 0.0001) and pFL-OPN (p < 0.001) were observed. Our results demonstrate that pFL-OPN reflect kidney injury among patients with leptospirosis.