Widespread amphotericin B-resistant strains of Aspergillus fumigatus in Hamilton, Canada
Widespread amphotericin B-resistant strains of Aspergillus fumigatus in Hamilton, Canada
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DOI：
10.2147/idr.s170952
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发表时间：
2018-01-01
影响因子：
3.9
通讯作者：
Xu, Jianping
中科院分区：
文献类型：
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作者：
Ashu, Eta E.;Korfanty, Gregory A.;Xu, Jianping
目的：两性霉素B(AMB)是治疗曲霉病的主要抗真菌药物之一，特别推荐用于治疗三唑耐药的黑曲霉。然而，世界上许多地区对烟曲霉菌的AMB敏感性模式知之甚少。本研究旨在描述加拿大安大略省汉密尔顿地区AMB的药敏模式。方法：采用微量肉汤稀释法测定195株环境和临床分离的烟曲霉菌对AMB的体外敏感性。结果：195株细菌中，188株(96.4%)的最低抑菌浓度(MIC)为2 mg/L，约80%和20%的临床分离株和环境分离株对AMB的最低抑菌浓度(MIC)为4 mg/L(P<0.05)。
Purpose: Amphotericin B (AMB) is one of the major antifungal drugs used in the management of aspergillosis and is especially recommended for treating triazole-resistant strains of Aspergillus fionigatus. However, relatively little is known about the AMB susceptibility patterns of A. fumigatus in many parts of the world. This study aims to describe the AMB susceptibility patterns in Hamilton, Ontario, Canada.Methods: The in vitro susceptibilities of 195 environmental and clinical A. fumigatus isolates to AMB were tested by the broth microdilution method as per the Clinical and Laboratory Standards Institute's guidelines. Catalase-generated oxygen bubbles trapped by Triton X-100 were used to quantify catalase activity in a representative group of isolates.Results: Of the 195 isolates, 188 (96.4%) had the minimum inhibitory concentration (MIC) of AMB >= 2 mg/L, with approximately 80% and 20% of all clinical and environmental isolates having MICs of >= 4 mg/L. Overall, the clinical isolates were less susceptible to AMB than environmental isolates (P-value