Secretion of cytokines by human macrophages upon infection by pathogenic and non-pathogenic mycobacteria
Secretion of cytokines by human macrophages upon infection by pathogenic and non-pathogenic mycobacteria
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DOI：
10.1006/mpat.1999.0345
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发表时间：
2000-05-01
影响因子：
3.8
通讯作者：
Rastogi, N
中科院分区：
文献类型：
--
作者：
Beltan, E;Horgen, L;Rastogi, N
The interaction of various pathogenic (Mycobacterium tuberculosis, M. avium, M. kansasii, M. xenopi), and non-pathogenic mycobacteria (M. smegmatis, M. phlei) with human macrophages at the level of macrophage cytokine expression (TNF alpha, IL1, IL6 and GM-CSF) was investigated. Both for TNF alpha and GM-CSF, the lowest levels were obtained with pathogenic mycobacterial species, whereas about 2-8 times higher levels were observed for non-pathogenic species, Contrary to the above, the differences for IL6 and IL1 were not marked, although IL6 appeared to be more elevated for non-pathogenic species. Heat-killed bacteria induced a lower level of the cytokines for all the three cytokines assayed (TNF alpha, IL6 and IL1), except for M. tuberculosis for whom a significantly higher proportion of TNF alpha was induced by killed bacilli. The RT-PCR experiments performed on M. avium (as a low inducer of the cytokines) and M. smegmatis (as a high inducer of the cytokines) showed that the differences observed among pathogenic vs non-pathogenic strains were also reflected at the transcriptional level for TNF alpha and to a lesser extent for IL6, but not for IL1. This investigation underlined important differences existing between the pathogenic and non-pathogenic species, particularly as regards TNF alpha and GM-CSF. (C) 2000 Academic Press.
